A kind of preparation method of aquaculture water oxygen enhancer
A technology of aquaculture water body and oxygenation agent, which is applied in the field of preparation of aquaculture water body oxygenation agent, can solve the problems of slow reduction of chemical oxygen consumption, poor oxygenation effect, short effective time, etc. Growth space, the effect of increasing dissolved oxygen
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[0029] A preparation method of an oxygenating agent for aquaculture water, comprising the steps of:
[0030] (1) Take the raw materials and add them to the enrichment medium at a mass ratio of 1:10, then add liquid paraffin with 10-12% of the mass of the enrichment medium, and culture at 28-33°C for 2-3 days. Line-inoculate into the separation medium and culture at 28-35°C for 15-18 hours, pick the colony with the largest diameter and inoculate it repeatedly into the separation medium for 2-3 times to obtain purified colonies, and take the fish and shrimp feed according to the mass ratio of 1 : 100, add sterile water, keep at 40~50°C for 1~2 days, filter, take the filtrate, inoculate the purified colonies into the filtrate at 3% inoculum size, and incubate at 35~37°C, 200 rpm for 18~24 h , repeat the culture 2~3 times, take the culture solution and spread it on the casein medium, incubate at 35~37°C for 18~24 h, pick the three single colonies with the largest diameter and inoc...
Embodiment 1
[0035] Fish and shrimp feed: purchased from Guangdong Hengxing Feed Co., Ltd., which can meet the feeding needs of fish and shrimp in the growth period.
[0036] Raw material: It comes from the bottom mud of fish and shrimp breeding ponds in Taihu Lake.
[0037] Enrichment medium: in parts by mass, take 1 part of ammonium chloride, 0.2 part of magnesium chloride, 0.5 part of dipotassium hydrogen phosphate, 2 parts of sodium chloride, 50 parts of sodium bicarbonate, 20 parts of acetone with a mass fraction of 4%. Amino acid, adjust the pH to 7.0, and sterilize at 121°C for 20min.
[0038] Separation medium: in parts by mass, take 1 part of ammonium chloride, 0.2 parts of magnesium chloride, 2 parts of yeast extract, 0.5 parts of dipotassium hydrogen phosphate, 2 parts of sodium chloride, 20 parts of agar, 1000 parts of water, and extinguish at 121 °C Bacteria 20min.
[0039] Feed medium: take fish and shrimp feed, add sterile water and agar at a mass ratio of 1:100:20, pH 7.0...
Embodiment 2
[0048] Fish and shrimp feed: purchased from Guangdong Hengxing Feed Co., Ltd., which can meet the feeding needs of fish and shrimp in the growth period.
[0049] Raw material: It comes from the bottom mud of fish and shrimp breeding ponds in Taihu Lake.
[0050] Enrichment medium: in parts by mass, take 1 part of ammonium chloride, 0.3 part of magnesium chloride, 0.6 part of dipotassium hydrogen phosphate, 2 parts of sodium chloride, 55 parts of sodium bicarbonate, and 25 parts of acetone with a mass fraction of 4%. Amino acid, adjust the pH to 7.0, and sterilize at 121°C for 20min.
[0051] Separation medium: in parts by mass, take 1 part of ammonium chloride, 0.3 parts of magnesium chloride, 2 parts of yeast extract, 0.7 parts of dipotassium hydrogen phosphate, 2 parts of sodium chloride, 23 parts of agar, 1000 parts of water, and extinguish at 121 °C Bacteria 20min.
[0052] Feed medium: take fish and shrimp feed, add sterile water and agar at a mass ratio of 1:100:20, pH...
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