Citric acid cross-linked chitosan hydrogel and preparation method thereof

A technology of cross-linking chitosan and citric acid, which is applied in the field of hydrogel preparation, can solve the problems of no treatment, irreversible damage to sensorimotor function, and affect the quality of life of patients, and achieve the effect of strengthening the cross-linking strength

Active Publication Date: 2018-07-31
WUHAN UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Spinal cord injury (SCI) is one of the common serious clinical diseases. Mechanical injuries such as car accidents, sports, violence, etc. all lead to spinal cord injuries, and spinal cord injuries can cause irreversible damage to the sensorimotor function of the central nervous s

Method used

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  • Citric acid cross-linked chitosan hydrogel and preparation method thereof
  • Citric acid cross-linked chitosan hydrogel and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 0.6g chitosan is dissolved in the 1% acetic acid solution of 20ml to obtain the chitosan solution of 3wt%, dissolve under magnetic stirring, be warming up to 40 ℃, add 0.1g citric acid, citric acid and chitosan in the gained solution The mass ratio was 1 / 6, stirred for 1 hour, then poured the blended solution into the mold for ultrasonic vibration to remove the air bubbles inside, frozen at -20°C for 24 hours, and then thawed at 20°C for 12 hours to obtain the initial gel. Prepare MES solution at pH 4, add EDC and NHS at 4 °C. Then put the initial gel into the MES solution of EDC / NHS, react at 4°C for 12h to obtain the citric acid cross-linked chitosan hydrogel, take out the gel, and put them in water, ethanol and NaOH solution with pH 8 respectively Washing three times yields the final hydrogel.

[0033] Such as figure 1 , showing the infrared spectrogram of the hydrogel in Example 1 of the present invention, the formation of amide bonds can be observed, indicating t...

Embodiment 2

[0035] 0.6g chitosan is dissolved in the 1% acetic acid solution of 20ml to obtain the chitosan solution of 3wt%, dissolve under magnetic stirring, be warming up to 70 ℃, add 0.05g citric acid, citric acid and chitosan in the gained solution The mass ratio was 1 / 12, stirred for 1 hour, then poured the blended solution into a mold for ultrasonic vibration to remove air bubbles, frozen at -80°C for 8 hours, and then thawed at 40°C for 8 hours to obtain the initial gel. Prepare a MES solution with a pH of 7, add EDC and NHS at 4°C, then put the primary gel into the MES solution of EDC / NHS, react at 4°C for 12h to obtain a citric acid cross-linked chitosan hydrogel, The gel was taken out and washed three times in water, ethanol and NaOH solution with pH 11 respectively to obtain the final hydrogel.

[0036] Such as figure 2 As shown, it can be observed that the hydrogel has a porous macroporous structure.

Embodiment 3

[0038] 0.6g chitosan is dissolved in the 1% acetic acid solution of 20ml to obtain the chitosan solution of 3wt%, dissolve under magnetic stirring, be warming up to 40 ℃, add 0.1g citric acid, citric acid and chitosan in the gained solution The mass ratio was 1 / 6, stirred for 1 hour, then poured the blended solution into the mold for ultrasonic vibration to remove the air bubbles inside, frozen at -20°C for 24 hours, and then thawed at 20°C for 12 hours to obtain the initial gel. Then it is added into the genipin solution to carry out the amide cross-linking reaction to obtain the citric acid cross-linked chitosan hydrogel. Washing three times in water, ethanol, and pH 8 NaOH solution, respectively, yielded the final hydrogel.

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Abstract

The invention relates to citric acid cross-linked chitosan hydrogel and a preparation method thereof. The preparation method comprises the following steps of dissolving chitosan in an acetic acid solution, heating, then adding citric acid, subsequently, carrying out freezing and unfreezing, so that a preliminary hydrogel model is obtained, and afterwards, carrying out an amidation cross-linking reaction on the hydrogel model, so as to finally obtain the hydrogel which is porous and macroporous and is in different shapes. According to the preparation method, the chitosan is cross-linked by utilizing the citric acid; the hydrogel which is favorable in biocompatibility and is used for repairing spinal cord injury is obtained, and the hydrogel is used for the spinal cord injury, can be used for inhibiting the formation of a scar and can be used for lightening the inflammatory reaction of a secondary injury after the spinal cord injury.

Description

technical field [0001] The invention belongs to the field of hydrogel preparation, in particular to a preparation method of citric acid cross-linked chitosan hydrogel. Background technique [0002] Spinal cord injury (SCI) is one of the common serious clinical diseases. Mechanical injuries such as car accidents, sports, violence, etc. all lead to spinal cord injuries, and spinal cord injuries can cause irreversible damage to the sensorimotor function of the central nervous system of patients, seriously affecting patients. However, there is no effective treatment at home and abroad, and the selection of biological implant materials to provide a good regenerative microenvironment for spinal cord injury is one of the research directions. [0003] Chitosan is the only alkaline polysaccharide in nature. It is a natural molecular material obtained by deacetylation of chitin. It has good biocompatibility, biodegradability, antioxidant activity, antibacterial properties and antitumo...

Claims

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Application Information

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IPC IPC(8): C08J3/24C08J9/00C08L5/08
CPCC08J3/24C08J9/00C08J2305/08
Inventor 戴红莲刘坤李雯雯
Owner WUHAN UNIV OF TECH
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