Application and method of 5-formyltetrahydrofolate in improving nitrogen utilization rate of plants

A technology of leucovorin and 5-F-THF, which is applied in the agricultural field, can solve the problems of excessive nitrogen fertilizer application and low utilization efficiency, and achieves the improvement of growth condition, the increase of amino acid content, the increase of fresh weight and chlorophyll content. Effect

Inactive Publication Date: 2021-02-23
THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to solve the problems of excessive nitrogen fertilizer application and low utilization efficiency in the prior art, the present invention provides the application and method of 5-formyltetrahydrofolate in improving plant nitrogen utilization efficiency

Method used

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  • Application and method of 5-formyltetrahydrofolate in improving nitrogen utilization rate of plants
  • Application and method of 5-formyltetrahydrofolate in improving nitrogen utilization rate of plants
  • Application and method of 5-formyltetrahydrofolate in improving nitrogen utilization rate of plants

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Effect test

Embodiment 1

[0017] 5-Methyltetrahydrofolate (5-M-THF) and 5-formyltetrahydrofolate (5-F-THF) are the two main forms of folic acid in plants. The invention isolates and obtains a folic acid mutant, the main form of folic acid in the folic acid mutant is 5-M-THF, and its content is obviously reduced, and the folic acid mutant shows a phenotype of shortened main root.

[0018] The present invention applies 500 μM 5-M-THF exogenously to the folic acid mutant, such as Figure 8 As shown, the folic acid mutant still maintains the phenotype of tap root development defects; when 500 μM 5-F-THF is applied exogenously to the folic acid mutant, the development of the mutant's tap root is improved, and the tap root length returns to the wild-type level. Therefore, it was shown that 5-F-THF could enter and be utilized in Arabidopsis seedlings.

Embodiment 2

[0020] Using Arabidopsis thaliana and rice as materials, the effects of exogenous application of 5-formyltetrahydrofolate (5-F-THF, 5-formyl-tetrahydrofolate) on alleviating low nitrogen stress in seedlings were analyzed.

[0021] experiment procedure:

[0022] The 5-formyltetrahydrofolate powder was prepared into a 10mM stock solution with sterilized water, and stored at -80°C for future use.

[0023] Arabidopsis thaliana, Columbia-0 wild-type seeds (Arabidopsis thaliana, Columbia-0) were sown on the medium supplemented with 5-formyltetrahydrofolate (working concentration 500 μM) after surface disinfection, and the medium only used NO 3- As the only nitrogen source, other components are the same as 1 / 2MS medium. NO in 9.4N medium 3- The concentration is 9.4mM; NO in 0.3N medium 3- The concentration is 0.3mM, while adding 9.1mM KCl to make K + The concentration was maintained at 9.4 mM. The Arabidopsis plate was placed in a light incubator, the culture conditions were set...

Embodiment 3

[0032] experiment procedure:

[0033] The 5-formyltetrahydrofolate powder was prepared into a 10mM stock solution with sterilized water, and stored at -80°C for future use.

[0034] Arabidopsis thaliana, Columbia-0 wild-type seeds (Arabidopsis thaliana, Columbia-0) were sown on a medium supplemented with 5-formyltetrahydrofolate after surface disinfection, and the medium was only used with NO 3- As the only nitrogen source, other components are the same as 1 / 2MS medium. NO in 9.4N medium 3- The concentration is 9.4mM; NO in 0.3N medium3- The concentration is 0.3mM, while adding 9.1mM KCl to make K + The concentration was maintained at 9.4 mM. The Arabidopsis plate was placed in a light incubator, the culture conditions were set at 22°C, the photoperiod was 16 h light / 8 h dark, and the light intensity was 60 μE m -2 the s -1 . On the 10th day of light culture, the materials were collected, washed with sterilized ultrapure water, and the folic acid extraction and content d...

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Abstract

The invention belongs to the field of agricultural technology, and in particular relates to the application of 5-formyltetrahydrofolate in improving plant nitrogen utilization. Exogenous 5‑F‑THF promotes the growth and development of plant seedlings under low nitrogen conditions, and the nitrogen metabolism of seedlings changes, which is helpful for understanding the biological functions of 5‑F‑THF in plants and improving plant nitrogen utilization in agricultural production Efficiency matters.

Description

technical field [0001] The invention belongs to the technical field of agriculture, and in particular relates to the application and method of 5-formyltetrahydrofolate in improving plant nitrogen utilization. Background technique [0002] Nitrogen is one of the important mineral elements necessary for plant growth and development. It is an essential element for the synthesis of amino acids, and then for the synthesis of proteins, nucleotides, chloroplasts and other metabolites and cell components. The nitrogen that plants absorb from the soil is mainly inorganic nitrogen at first, and can also absorb organic nitrogen, such as urea. Inorganic nitrogen mainly includes nitrate nitrogen (NO 3- ) and ammonium nitrogen (NH 4+ ). For most plants, nitrate nitrogen (NO 3- ) is the most important form of nitrogen source. Plants use nitrate nitrogen through three main processes: absorption, reduction and assimilation. The nitrogen of nitrate is in a highly oxidized state, which n...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/90A01P21/00
CPCA01N43/90
Inventor 张春义梁秋菊易尘
Owner THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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