Method for crude ganoderma lucidum spore powder wall shell polysaccharide through nano pulverization
A Ganoderma lucidum spore powder and nano-crushing technology, which is applied in the field of extracting effective substances from fungi, can solve problems such as difficulty in obtaining effective substances, achieve the effects of reducing difficulty, increasing active polysaccharide content, and excellent moisturizing effect
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Embodiment 1
[0048] Composition determination of embodiment 1 Ganoderma lucidum spore powder wall shell waste
[0049] 1. Determination of moisture and ash content: Take samples No. 1 and No. 2 respectively, and conduct moisture determination according to GB / T 12531-1990 Moisture Standard for Edible Fungi, and measure ash content according to GB 12532-1990 Determination Standard for Ash Content of Edible Fungi.
[0050] The results showed that the moisture contents of samples No. 1 and No. 2 were 8.20%, 14.8%, and the ash contents were 0.99%, 1.04%, respectively.
[0051] 2. Determination of protein and amino acid content: Samples No. 1 and No. 2 were taken respectively, and the protein content was determined according to the GB 5009.5-2010 method, and the amino acid content was determined according to the GB / T 5009.124-2003 method.
[0052] The results showed that the total protein contents of No. 1 and No. 2 samples were 24.58% and 23.08%, and the contents of amino acids were 7.04% and 6...
Embodiment 2
[0061] The effect of this test on the content of active polysaccharides by nano-grinding treatment
[0062] Take 500 g of sample No. 1 and sample No. 2 respectively, and pulverize them with a nano pulverizer at a pulverization temperature of 20° C., a pulverization time of 10 h, a nano pulverization power of 4 kw, and a pulverization particle size of 800 mesh to obtain waste nanopowder.
[0063] The content of active polysaccharides in the obtained waste nano-powder was determined by the phenol-sulfuric acid method, and the results were 4.77% and 5.15% respectively. As can be seen from the results, the present invention can significantly increase the content of active polysaccharides in the extraction waste of Ganoderma lucidum spore powder by adopting the nano-grinding method, so that the content of Ganoderma lucidum polysaccharides that can be extracted reaches more than 4%, providing sufficient for subsequent extraction steps extractable substances.
[0064] Compared with ...
Embodiment 3
[0066] Weigh 500g of Ganoderma lucidum spore powder extraction waste No. 1 sample, and conduct nano-grinding under the conditions of 15°C, nano-grinding power of 4kw, and milling time of 12 hours to obtain 500g of waste nano-powder with a particle size of 600-750 mesh. Take 100g of waste nanopowder and mix with 3.5L of distilled water, extract at 100°C for 2 hours, centrifuge, and filter to obtain the supernatant, which is the crude polysaccharide extract.
[0067] Concentrate the obtained crude polysaccharide extract under reduced pressure to 0.1L at 100mbar and 50°C, freeze the concentrated liquid at -20°C for 2 hours, then freeze at -80°C for 3 hours, and put it in a freeze dryer Freeze-dried to obtain 4.12 g of crude polysaccharides from the wall shell of Ganoderma lucidum spore powder.
[0068] The polysaccharide content of the prepared Ganoderma lucidum spore powder wall shell crude polysaccharide was measured, the method was as the polysaccharide content determination s...
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