Method for preparing panax notoginseng polysaccharide with high anti-aging activity by utilizing irradiation

A technology of notoginseng polysaccharide and irradiation, which is applied in the direction of anti-toxic agent and drug combination, can solve the problem that it is difficult to obtain the content of notoginseng polysaccharide, and achieve excellent anti-aging effect, low irradiation processing dosage and high yield Effect

Active Publication Date: 2022-05-13
SICHUAN INST OF ATOMIC ENERGY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to improve the added value of Panax notoginseng and solve the problem that it is difficult to obtain polysaccharides from Panax notoginseng and its content is low from the dregs of Panax notoginseng after extracting saponins, the invention provides a high anti-aging polysaccharide with an active polysaccharide content of more than 70%. The preparation method of active Panax notoginseng polysaccharide not only reduces environmental pollution and waste of resources, but also further develops the anti-oxidation and anti-aging medicinal value of Panax notoginseng polysaccharide through irradiation technology

Method used

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  • Method for preparing panax notoginseng polysaccharide with high anti-aging activity by utilizing irradiation
  • Method for preparing panax notoginseng polysaccharide with high anti-aging activity by utilizing irradiation
  • Method for preparing panax notoginseng polysaccharide with high anti-aging activity by utilizing irradiation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Take 4.56g of ammonium persulfate, dissolve it in 100ml of water, and spray it onto 1000g of Panax notoginseng medicinal residues. After 30kGy of electron beam irradiation, add 15L of water for 80W ultrasonic extraction for 3h, collect the extract and concentrate it to 1.5L under reduced pressure. Hydrochloric acid and citric acid were added to the solution to adjust the pH to 4.5, and after filtering out insoluble matter such as macromolecular protein impurities, 4.5 L of absolute ethanol was added to the filtrate, and the polysaccharide precipitate obtained by alcohol precipitation was frozen at -10 ° C for 4 hours, and the obtained pre The frozen material was then sent to a freeze dryer and freeze-dried at a temperature of -75°C and a vacuum of 0.8mbar to obtain 87.5g of Panax notoginseng polysaccharide with an active polysaccharide content of 72.89%, with a yield of 6.38%; measured by gel permeation chromatography Its molecular weight is 185.4kD, which is notoginseng...

Embodiment 2

[0033] Take 3.42g of ascorbic acid, dissolve it in 100ml of water and spray it onto 1000g of Panax notoginseng medicinal residues. After 50kGy of electron beam irradiation, add 15L of water for 100W ultrasonic extraction for 2h, collect the extract and concentrate it to 1.5L under reduced pressure. Adjust the pH to 4.5 with acetic acid, filter out insoluble matter such as macromolecular protein impurities, add 4.5L of 95% ethanol to the filtrate, freeze the polysaccharide precipitate obtained by alcohol precipitation at -10°C for 4 hours, and then send the pre-frozen material to the freezer Freeze-dried in a dryer at a temperature of -80°C and a vacuum of 0.8mbar to obtain 108.2g of Panax notoginseng polysaccharide with an active polysaccharide content of 76.25%, with a yield of 8.25%; the molecular weight was 138.4kD as measured by gel permeation chromatography , which is notoginseng polysaccharide with high anti-aging activity.

Embodiment 3

[0035] Take 5g of potassium persulfate, dissolve it in 100ml of water and spray it onto 1000g of Panax notoginseng medicinal residues. After electron beam irradiation of 150kGy, add 10L of water for 50W ultrasonic extraction for 3h, collect the extract and concentrate it to 2L under reduced pressure, and add the concentrate to Adjust the pH to 4.5 with citric acid, filter out insoluble matter such as macromolecular protein impurities, add 6L of absolute ethanol to the filtrate, freeze the polysaccharide precipitate obtained by alcohol precipitation at -15°C for 3 hours, and then send the pre-frozen material to the freezer Freeze-dried in a dryer at a temperature of -70°C and a vacuum of 1.0mbar to obtain 186.5g of Panax notoginseng polysaccharide with an active polysaccharide content of 72.71%, with a yield of 13.56%; the molecular weight was 45.8kD as measured by gel permeation chromatography , which is notoginseng polysaccharide with high anti-aging activity.

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Abstract

The invention belongs to the technical field of irradiation and extraction of active ingredients of traditional Chinese medicines, and particularly discloses a method for preparing panax notoginseng polysaccharide with high anti-aging activity by utilizing irradiation, which comprises the following steps: spraying an irradiation sensitizer solution on panax notoginseng waste residues, and irradiating by electron beams until the dose is 30kGy-200kGy to obtain irradiated panax notoginseng residues; mixing the irradiated pseudo-ginseng dregs with water, and performing heating or ultrasonic extraction to obtain a pseudo-ginseng polysaccharide extracting solution; and concentrating the extracting solution under reduced pressure, adding acid to adjust the pH value to 4.5, adding ethanol into the filtrate, and freeze-drying the polysaccharide precipitate subjected to alcohol precipitation to obtain the panax notoginseng polysaccharide with the active polysaccharide content of more than 70%. The molecular weight of the panax notoginseng polysaccharide can be precisely regulated and controlled, compared with panax notoginseng polysaccharide processed without irradiation, the content of active polysaccharide is increased by 2-3 times or above, the processing capacity is large, the yield is high, energy consumption is low, the panax notoginseng polysaccharide is clean and free of pollution, large-scale industrial production can be achieved, and the antioxidant and anti-aging medicinal value of the panax notoginseng polysaccharide is further developed through the irradiation technology.

Description

technical field [0001] The invention belongs to the technical field of irradiation and extraction of active ingredients of traditional Chinese medicines, and in particular relates to a method for preparing Panax notoginseng polysaccharide with high anti-aging activity by irradiation technology. Background technique [0002] Panax notoginseng polysaccharide can promote and strengthen the function of reticuloendothelial system, enhance immunity, resist microwave radiation, promote the recovery of trauma and promote the repair of bone defects, etc., and has gradually attracted widespread attention. At present, there are more than 400 varieties of Chinese patent medicines with Panax notoginseng as raw materials, 3,200 approval numbers, and 1,300 manufacturers involved. With the continuous expansion of social development, the annual planting area of ​​Panax notoginseng is about 100,000 mu, with an output of 9,000 Ton. The Panax notoginseng used to extract the total saponins of P...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P39/06
CPCC08B37/0003A61P39/06
Inventor 张晓彬倪茂君王静霞彭朝荣黄敏张婧刘思阳陈屿恒先丽高鹏
Owner SICHUAN INST OF ATOMIC ENERGY
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