Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing crude ganoderma lucidum spore powder wall shell polysaccharide through irradiation

A technology of Ganoderma lucidum spore powder and crude polysaccharide, which is applied in the field of extracting effective substances of fungi, can solve the problems such as difficulty in obtaining effective substances, and achieve excellent immune regulation function, moisturizing effect, and excellent moisturizing effect

Active Publication Date: 2018-10-09
JINHUA SHOUXIANGU PHAMACEUTICAL CO LTD +1
View PDF2 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem that it is difficult to obtain effective substances in the extraction waste of Ganoderma lucidum spore powder after water extraction and degreasing, the present invention provides a method for preparing Ganoderma lucidum spore polysaccharide from the extraction waste of Ganoderma lucidum spore powder with an active polysaccharide content below 1%. method

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing crude ganoderma lucidum spore powder wall shell polysaccharide through irradiation
  • Method for preparing crude ganoderma lucidum spore powder wall shell polysaccharide through irradiation
  • Method for preparing crude ganoderma lucidum spore powder wall shell polysaccharide through irradiation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Composition determination of embodiment 1 Ganoderma lucidum spore powder wall shell waste

[0046] 1. Determination of moisture and ash content: Take samples No. 1 and No. 2 respectively, and conduct moisture determination according to GB / T 12531-1990 Moisture Standard for Edible Fungi, and measure ash content according to GB 12532-1990 Determination Standard for Ash Content of Edible Fungi.

[0047] The results showed that the moisture contents of samples No. 1 and No. 2 were 8.20%, 14.8%, and the ash contents were 0.99%, 1.04%, respectively.

[0048] 2. Determination of protein and amino acid content: Samples No. 1 and No. 2 were taken respectively, and the protein content was determined according to the GB 5009.5-2010 method, and the amino acid content was determined according to the GB / T 5009.124-2003 method.

[0049] The results showed that the total protein contents of No. 1 and No. 2 samples were 24.58% and 23.08%, and the contents of amino acids were 7.04% and 6...

Embodiment 2

[0058] This experiment verified the effect of irradiation treatment on polysaccharide content

[0059] Take 500g of sample No. 1 and sample No. 2 respectively, and irradiate them with electron beams to a dose of 500kGy to obtain irradiated waste;

[0060] The polysaccharide content was measured by the phenol-sulfuric acid method, and the specific operation was the same as the polysaccharide content determination step in Example 1. The measurement results showed that the polysaccharide content in the irradiated waste prepared by No. 1 sample and No. 2 sample was 7.78% and 6.38% respectively. %.

[0061] As can be seen from the results, after the extraction waste of ganoderma lucidum spore powder was irradiated, the water-soluble polysaccharide content therein was significantly improved. Compared with the polysaccharide content measured in Example 1, the irradiated waste in the irradiation waste The polysaccharide content is increased by more than 8 times, which is more than 3 ...

Embodiment 3

[0063] Weigh 500 g of sample No. 1 of Ganoderma lucidum spore powder extraction waste, and irradiate it with an electron beam to a dose of 500 kGy to obtain irradiated waste. Mix 100g of irradiated waste with 4L of distilled water, extract at 100°C for 2 hours, centrifuge, and filter to obtain the supernatant, which is the crude polysaccharide extract.

[0064] Concentrate the obtained crude polysaccharide extract under reduced pressure to 0.1L at 100mbar and 50°C, freeze the concentrated liquid at -20°C for 2 hours, then freeze at -80°C for 3 hours, and put it in a freeze dryer Freeze-drying to obtain 7.03 g of crude polysaccharide of ganoderma lucidum spore powder.

[0065] The polysaccharide content of the prepared Ganoderma lucidum spore powder wall shell crude polysaccharide was determined, and the method was as described in Example 1. The results showed that the active polysaccharide content was 78.59%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
The molecular weight distributionaaaaaaaaaa
Login to View More

Abstract

The invention relates to the technical field of fungi, and provides a method for preparing crude ganoderma lucidum spore powder wall shell polysaccharide through irradiation. The method comprises thefollowing steps: performing irradiation treatment on ganoderma lucidum spore powder extraction waste, and irradiating by an electron beam till the dosage of 500-900kGy to obtain irradiated waste; mixing the irradiated waste and water, heating for extracting, and performing solid-liquid separation to obtain a crude polysaccharide extraction solution; drying the crude polysaccharide extraction solution to obtain the crude ganoderma lucidum spore powder wall shell polysaccharide. The raw material for extraction is the ganoderma lucidum spore powder extraction waste in which the content of ganoderma lucidum polysaccharide is lower than 1% and the content of substances which can be dissolved in water and an organic reagent is extremely low. In the crude ganoderma lucidum spore powder wall shellpolysaccharide prepared by the method provided by the invention, the content of active polysaccharide is 78% or above; compared with ganoderma lucidum spore polysaccharide extracted from ganoderma lucidum spore powder as a raw material, the crude ganoderma lucidum spore powder wall shell polysaccharide also has the advantages of an excellent immunomodulatory function and an excellent moisturizingeffect; the crude ganoderma lucidum spore powder wall shell polysaccharide can be widely applied to the field of functional food, health-care food, medicines, cosmetics and the like.

Description

technical field [0001] The invention relates to the technical field of extraction of fungal effective substances, in particular to a method for preparing ganoderma lucidum spore powder wall shell crude polysaccharide by irradiation. Background technique [0002] Ganoderma lucidum spore powder is extremely tiny spores ejected from the gills of Ganoderma lucidum at the mature stage, and has all the genetically active substances of Ganoderma lucidum. Ganoderma lucidum spores are collected into fine powder, commonly known as Ganoderma lucidum spore powder, and its main components are polysaccharides, adenine, nucleotides, organic germanium, triterpenoids, ergosterol, vitamins, carotene and trace elements, etc. Tumor, hypoxia resistance, improve immunity and other effects. Among them, Ganoderma lucidum spore polysaccharide has a regulating effect on the human immune system, and can significantly increase the clearance index activity of cyclophosphamide immunosuppressed mice. Ga...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C08B37/00
CPCC08B37/00C08B37/0003
Inventor 王晓彤史月姣李振宇李振皓李明焱
Owner JINHUA SHOUXIANGU PHAMACEUTICAL CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com