Zirconia microbead rh blood type reagent card and preparation method thereof

A zirconia microbead and reagent card technology, applied in the field of medical testing, can solve the problems of affected centrifugal chromatography results, change of particle pore size, poor physical strength, etc., to avoid the increase of ionic strength, prevent the reproduction of bacteria, and buffer capacity. strong effect

Active Publication Date: 2021-02-09
广东睿碁生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Sephadex will be affected by changes in temperature, salt concentration, osmotic pressure, and pH. The pore size of the particles will change, and the space outside the bed will also change, which will affect the results of centrifugal chromatography.
Because it is a gel-like particle, its physical strength is poor, and it will cause deformation as the storage period prolongs, and its non-specific adsorption is high, which will affect the test results
In addition, the price is more expensive

Method used

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  • Zirconia microbead rh blood type reagent card and preparation method thereof
  • Zirconia microbead rh blood type reagent card and preparation method thereof
  • Zirconia microbead rh blood type reagent card and preparation method thereof

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Experimental program
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Embodiment 1

[0047] Embodiment 1: Preparation of zirconia microbeads Rh blood group typing reagent card

[0048] Step 1, preparation of zirconia microbead suspension medium

[0049] The formulation of the zirconia microbead suspension medium is as follows:

[0050]

[0051] Dissolve the above reagents in distilled water and adjust the pH value to 6.6-6.8.

[0052] Step 2, preparation of zirconia microbeads

[0053] Zirconia microspheres are selected, and the particle diameter is 63-125 microns. Wash 3-5 times with the zirconia microbead suspension medium prepared in step 1 to remove the broken fragments of zirconia microbeads and aggregated zirconia microbead particles, and collect suitable zirconia microbeads with uniform particle size and complete spherical shape. beads.

[0054] Step 3. Antibody selection

[0055] Choose a monoclonal anti-C antibody with IgM properties, with a titer ≥ 16;

[0056] Choose a monoclonal anti-c antibody with IgM properties, with a titer ≥ 16;

[005...

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Abstract

The invention provides a zirconia (ZrO 2 ) microbead Rh blood type reagent card and preparation method thereof, said reagent card comprising: zirconia microbead microcolumn tube containing monoclonal anti-C antibody of IgM property, zirconia microcolumn tube containing monoclonal anti-C antibody of IgM property Microbead microcolumn tube, zirconia microcolumn tube containing monoclonal anti-E antibody with IgM property, zirconia microbead microcolumn tube with monoclonal anti-E antibody with IgM property, monoclonal anti-D with IgM property Zirconia Beads Tubes for Antibodies and Zirconia Beads Tubes with Zirconia Beads Suspension Medium. The zirconia microbead Rh blood type reagent card of the invention has high sensitivity, good specificity and stable quality.

Description

technical field [0001] The invention relates to the field of medical detection. More specifically, the present invention relates to a zirconia microbead Rh blood type reagent card and a preparation method thereof. Background technique [0002] The Rh blood group is a blood group system first discovered in 1940. The discovery of the Rh blood type plays a very important role in guiding blood transfusion more scientifically, further improving the experimental diagnosis of hemolytic disease of the newborn and maintaining the health of mother and child. [0003] The Rh blood group system contains 6 antigens, namely C, c, D, d, E, e. Where erythrocytes contain D antigen, they are Rh positive; otherwise, they are negative. Rh blood type has no natural antibodies, and its antibodies are mostly produced by blood transfusion or pregnancy immunization, which has important clinical significance. Once antibodies are formed, severe transfusion reactions can occur if Rh-positive blood ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/80G01N33/531
CPCG01N33/531G01N33/80
Inventor 高晓玲阙秋华周辰杰贺诗香
Owner 广东睿碁生物科技有限公司
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