Nucleic acid composition, detection kit and method of use for detection of hereditary anemia
A technology for detection kits and nucleic acid compositions, applied in the fields of molecular biology and biomedicine, can solve problems such as unreasonable primer design, high homology, and limited detection throughput, so as to reduce the risk of missed detection of hereditary anemia and improve Accuracy and reliability, the effect of ensuring the quality of the amplification
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Embodiment 1
[0107] Using the hereditary anemia detection kit of the present invention and the method for using the above kit, detection and verification are performed on normal people and several clinical samples. Normal samples were from volunteers of the applicant unit, and clinical samples were from Foshan Maternal and Child Health Hospital (all samples were approved by the Ethics Committee of Foshan Maternal and Child Health Hospital, and the patient’s informed consent was obtained), and the sequencing method, etc. Identified as 41 / 42M / N, sickle cell anemia heterozygous, alpha WS α, -α 4.2 / αα and -α SEA / αα type of anemia. Figure 3 to Figure 8 Normal people, 41 / 42M / N, sickle cell anemia heterozygotes, α WS α, -α 4.2 / αα and -α SEA The test results of / αα can be seen from the figure, all the test results are consistent with the original identification results.
Embodiment 2
[0108] Example 2: Genome conditions and detection conditions extracted from samples from different sources
[0109] In order to analyze samples from different sources, such as samples from peripheral blood, saliva, urine, and blood film, use the corresponding genome extraction kit to extract the concentration, purity, and detection of genomic DNA. In this example, 5 volunteers were collected. Samples from different sources were compared. The genome extraction kits used are:
[0110] Whole blood genome extraction kit: use CWBIO's Blood Genomic DNA Midi Kit (1-5ml) (Cat. No. CW0541S);
[0111] Saliva DNA extraction kit: use EMI's BIOG saliva DNA extraction kit (Cat. No. 51043);
[0112] Urine DNA Extraction Kit: Use EMI’s BIOG Urine DNA Extraction Kit (Cat. No. 51045);
[0113] Blood film DNA extraction kit: use CWBIO's FTAcard DNA Kit (Cat. No. CW2275S).
[0114] The extracted genomic DNA is shown in Table 11 below (all DNA was dissolved in 50 μl ultrapure water).
[0115]...
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