A kind of effective part of madder and its preparation method and application

A technology of effective parts and madder, applied in the field of effective parts of madder and its preparation, can solve the problems of low immunization rate, easy mutation of influenza virus, difficulty in group immunization, etc., and achieves simple preparation process, strong antiviral activity, and antiviral properties. strong effect

Active Publication Date: 2021-09-03
JIANGSU KANION PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current main prevention and control measure for influenza virus is vaccination, but the vaccine itself has certain limitations: the immunization rate is low, it is difficult to produce effective herd immunity; it is difficult to produce effective immunity for high-risk groups; due to the aggregation of influenza virus RNA The enzyme lacks a post-translational correction mechanism, and influenza viruses are prone to mutations, so it is difficult to produce corresponding vaccines in a short period of time in the early stages of the rapid spread of newly mutated viruses
[0005] The main chemical components of Rubia plants are water-soluble cyclohexyl peptides, fat-soluble anthraquinone and its glycosides, reduced naphthoquinone and its glycosides, and also contain polysaccharides, terpenes, trace elements and sitosterol, madder However, there are very few research reports on the effective parts of rubia anthraquinone, let alone the relevant reports on the antiviral effect of rubia anthraquinone

Method used

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  • A kind of effective part of madder and its preparation method and application
  • A kind of effective part of madder and its preparation method and application
  • A kind of effective part of madder and its preparation method and application

Examples

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preparation example 1

[0029] Take the dried roots or rhizomes of Rubiaceae plant Rubiae, add 10 times the weight-to-volume ratio of water to decoct, decoct twice for 3 hours each time, combine the decoctions, filter, and concentrate the filtrate under reduced pressure to obtain medicinal solution 1. Dilute the obtained medicinal solution 1 with heated water, centrifuge to obtain a supernatant, and filter the obtained supernatant to obtain medicinal solution 2. The resulting filtrate 2 was extracted 3 times with ethyl acetate at a ratio of 1:3, the ethyl acetate layers were combined, and concentrated under reduced pressure to obtain a dry paste. After the dry paste was diluted with water, it was absorbed by an AB-8 macroporous resin column, and the effluent was discarded. , wash with water to remove water-soluble impurities, discard the washing liquid, then elute with 60% ethanol, collect the eluate, concentrate under reduced pressure, dry, and pulverize to obtain the antiviral effective part of madd...

preparation example 2

[0031] The dry root or rhizome of Rubiaceae plant Rubiae was added with 60% ethanol aqueous solution with a volume concentration of 8 times the weight to volume ratio, extracted 3 times for 2 hours each time, combined the extracts, filtered, and the filtrate was concentrated under reduced pressure to obtain medicinal solution 1. Dilute the obtained medicinal solution 1 with heated water, centrifuge to obtain a supernatant, and filter the obtained supernatant to obtain medicinal solution 2. The resulting filtrate 2 was extracted 3 times with n-butanol at a ratio of 1:4, the n-butanol layers were combined, and concentrated under reduced pressure to obtain a dry paste. After the dry paste was diluted with water, it was adsorbed by a HP-20 macroporous resin column, and the effluent was discarded. , wash with water to remove water-soluble impurities, discard the washing liquid, and then elute with 70% ethanol, collect the eluate, concentrate under reduced pressure, dry, and pulveriz...

preparation example 3

[0033] Add 70% ethanol aqueous solution with a volume concentration of 6 times the weight to volume ratio, extract 3 times, each time for 2 hours, combine the extracts, filter, and concentrate the filtrate under reduced pressure to obtain the medicinal solution 1. Dilute the obtained medicinal solution 1 with heated water, centrifuge to obtain a supernatant, and filter the obtained supernatant to obtain medicinal solution 2. The resulting filtrate 2 was extracted 3 times with ethyl acetate in a ratio of 1:5, the ethyl acetate layers were combined, and concentrated under reduced pressure to obtain a dry paste. After the dry paste was diluted with water, it was adsorbed by a D101 macroporous resin column, and the effluent was discarded. Wash with water to remove water-soluble impurities, discard the washing solution, elute with 80% ethanol, collect the eluate, concentrate under reduced pressure, dry, and pulverize to obtain the effective antiviral fraction of Rubia Cauliflower, w...

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Abstract

The invention discloses effective fractions of rubia chinensis and its preparation method and application. The effective part of madder with high anthraquinone content is obtained by using the preparation method of the extract of the present invention, the preparation process is simple, and the organic solvent is fully extracted, so that the anthraquinone content in the effective part of madder is more than 40%, easy to operate, and low in cost . Experiments show that the effective parts of madder obtained by this method have a very good antiviral effect, and then it is further found through experiments that the higher the total anthraquinone content, the stronger the antiviral effect, showing that the anthraquinones of madder have stronger antiviral effects. active.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicines, in particular to effective parts of rubia radiata and its preparation method and application. Background technique [0002] Influenza viruses can be divided into three types: A, B, and C according to the antigenicity of the core protein and matrix protein of the virus particle. Among them, influenza A virus can be divided into many subtypes according to the different structures of surface hemagglutinin (HA) and neuraminidase (NA) antigens. So far, 18 different subtypes of HA have been found, among which H1 and H3 can cause most influenza in humans, and H5, H7, and H9 can occasionally cause human infections. There are 11 subtypes of NA, and the main subtypes in humans are N1, N2 and N9. Influenza A virus is the main factor causing seasonal influenza in humans, and it can also infect a variety of animals including poultry, pigs, and horses. [0003] Influenza not only poses...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/74A61P31/12A61P31/16A61K31/7056
CPCA61K31/7056A61K36/74A61K2236/331A61K2236/333A61K2236/39A61K2236/51A61K2236/53A61K2236/55A61K2300/00
Inventor 萧伟李孟璇杨彪姜涛刘文君胡玉梅丁岗王振中
Owner JIANGSU KANION PHARMA CO LTD
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