Nucleic acid extractor and use method thereof

A nucleic acid extractor and nucleic acid technology, applied in the field of nucleic acid extraction, can solve the problem of inability to elute a large volume of samples to a small volume of purified products, and achieve the effect of improving the extraction effect and high nucleic acid concentration

Pending Publication Date: 2018-12-21
无锡百泰克生物技术有限公司
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Problems solved by technology

[0005] Purpose of the invention: In order to overcome the problem that the nucleic acid extractor in the prior art cannot complete the elution from large-volume samples to micro-volume purified products, provide a device that can achieve high-concentration nucleic acid by elution from large-volume samples to micro-volume purified products Nucleic acid extractor

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  • Nucleic acid extractor and use method thereof
  • Nucleic acid extractor and use method thereof

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Embodiment Construction

[0028] The present invention will be further explained below in conjunction with the accompanying drawings and specific embodiments.

[0029] In this embodiment, the feeding mechanism of the nucleic acid extractor is a disc rotation mechanism, which is equipped with a turntable 100, and the magnetic adsorption member on the magnetic attraction mechanism is selected as a magnetic rod.

[0030] Such as figure 1 and figure 2 As shown, the nucleic acid extractor includes a disc rotation mechanism, 6 deep-well plates and 5 magnetic suction mechanisms. The turntable 100 of the disc rotation mechanism is provided with 6 positioning seats 102, and the 6 deep-well plates are respectively embedded in 6 In the positioning groove of each positioning seat 102, the 6 deep well plates are respectively the first deep well plate 101, the second deep well plate 6, the third deep well plate 7, the fourth deep well plate 8, and the fifth deep well plate 9 and the sixth deep well plate 10, the ...

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Abstract

The invention discloses a nucleic acid extractor. Nucleic acid of a sample is extracted and concentrated simultaneously by a combined magnetic attraction mechanism. The nucleic acid extractor comprises a feeding mechanism, a plurality of deep pore plates and a plurality of magnetic attraction mechanisms, wherein magnetic adsorption pieces are arranged on the magnetic attraction mechanisms, the plurality of magnetic attraction mechanisms are correspondingly distributed on the feeding mechanism to form the combined magnetic attraction mechanism, and distribution positions of the magnetic attraction mechanisms are correspondingly matched with positions of the deep pore plates; the magnetic adsorption pieces with different sizes are arranged on different magnetic attraction mechanisms; the volumes of pore grooves in the deep pore plates are respectively correspondingly matched with the magnetic adsorption pieces with different sizes. By mutual cooperation of the combined magnetic attraction mechanism and the deep pore plates with a plurality of pore grooves with different volumes, processes of extraction, gradient rinsing and elution can be smoothly and sequentially completed, the elution process from a large-volume sample to a micro-volume purified product is realized, so that the concentration of finally obtained nucleic acid is very high, and the nucleic acid extraction effect is greatly improved.

Description

technical field [0001] The invention relates to the technical field of nucleic acid extraction, in particular to a nucleic acid extraction instrument and a method for using the same. Background technique [0002] Nucleic acid research is an important topic in modern biology and medical research. As the carrier of genetic information, nucleic acid is the material basis of gene expression. In addition to its important role in the normal growth, development and reproduction of organisms, it is also related to abnormal conditions of life, such as tumor occurrence, radiation damage, etc. , genetic diseases, etc. are also closely related. [0003] Nucleic acid extractor is an instrument that uses supporting nucleic acid extraction reagents to automatically complete the extraction of sample nucleic acid. Nucleic acid extractors are divided into two categories: one is large-scale automation, generally called automatic liquid workstation; the other is small The automatic nucleic ac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10
CPCC12N15/1013
Inventor 周志图
Owner 无锡百泰克生物技术有限公司
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