A kind of cultivation method of very early maturing rice
A breeding method and rice technology, which is applied in the field of crop genetics and breeding, can solve the problems of restricting popularization and application, inconsistencies in the maturity period of the whole plant of the main panicle and early maturing materials, and restricting cultivation.
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Embodiment 1
[0029] Embodiment 1: Utilize hybridization method to create oligo-tiller early-maturing rice material
[0030] Using a short-growing rice mutant sgp(t) (characterized by early maturation of the main panicle) (Liu Huaqing et al. (2008), Science Bulletin, 53(13):1552-1559, image 3 ) was hybridized with the oligo-tiller rice variety Nanchanghui, the hybrid F1 was self-crossed after planting, F2 seeds were obtained, and a F2 population of 1500 plants was planted; the heading stage of the main panicle, tillering, and maturity of the whole plant were investigated according to a single plant, and other agronomic traits were investigated in combination. Select plants with compact and upright leaves, plant height less than 1m, seed setting rate of more than 85%, main panicle sowing start within 50 days, oligotillers (total number of panicles ≤ 3), and whole plant maturity within 90 days to harvest as a single plant , planted into F3 strains; then select and plant F4, F5, F6 according ...
Embodiment 2
[0031] Example 2: Using genome editing technology to create oligo-tiller early-maturing rice materials
[0032] 1. Genome editing target site selection and vector construction
[0033] Selection of rice ideal plant type gene IPA1, using PCR to amplify short growth period mutant sgp(t) background IPA1 Gene sequence, after sequencing, it was found that its IPA1 The gene sequence is consistent with that of Nipponbare. According to the principle of CRISPR / CAS9 target site design, two target sites were selected in the miRNA156 binding site area, and their sequences were:
[0034] CIPA1-1, TCTCTTCTGTCAACCCAGCCATGG;
[0035] CIPA1-2, TCTCTCTCTTCTGTCAACCCAGC.
[0036] The backbone of the plant Cas9 / gRNA plasmid (VK005-01 Cas9 / gRNAVector) provided by Beijing Weishang Lide Company was used, which already included the 35S promoter and its hygromycin resistance gene, the Ubi promoter and its The rice Cas9 protein that has been optimized and the rU6 promoter that starts the gRNA als...
Embodiment 3
[0049] Embodiment 3: oligo-tiller early-maturing rice variety cultivation
[0050] Utilize the early-maturing oligo-tiller rice intermediate material created in Example 1 fte Hybrid rice varieties Minghui 86 and 93-11 were crossed, the hybrid F1 was backcrossed with the corresponding Minghui 86 and 93-11 respectively, and the backcross generation 1 (BC1) plants with early heading and oligotillers were selected to continue backcrossing with the recurrent parents. The 5th generation of backcross (BC5) plants were self-crossed to obtain homozygous oligotiller early-maturing rice varieties Zaomaming Minghui 86 (E86) and Zaoma 93-11 (E9311) with the background of recurrent parents. Their growth periods were 76 days and 78 days.
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