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Method for detecting fecundity of alpine Merino sheep based on INHA gene

A technology for merino sheep and genetic testing, applied in the field of genetic engineering, can solve problems such as affecting the reproductive ability of alpine merino sheep

Active Publication Date: 2019-01-25
LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the prior art has not reported which site of this gene can affect the reproductive ability of Alpine Merino sheep

Method used

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  • Method for detecting fecundity of alpine Merino sheep based on INHA gene
  • Method for detecting fecundity of alpine Merino sheep based on INHA gene
  • Method for detecting fecundity of alpine Merino sheep based on INHA gene

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Experimental program
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Embodiment 1

[0038] 1 Materials and methods

[0039] 1.1 Materials

[0040] 1.1.1 Sample collection

[0041] Gansu Province Sheep Reproductive Technology Promotion Station collected blood samples from 94 alpine Merino sheep (45 single-born ewes, 49 twin-born ewes), and collected 10 mL of blood from each sheep in blood collection tubes added with EDTA-K2 anticoagulant After the blood sample was collected, it was shaken and mixed quickly, put into a sampling box containing ice packs for temporary storage, and then transported back to the laboratory and stored in a -20°C refrigerator for DNA extraction.

[0042] 1.1.2 Main reagents and instruments

[0043] EDTA-K2 vacuum blood collection tubes were purchased from Jiangsu Yuli Medical Instrument Co., Ltd.; blood genome extraction kits were purchased from OMEGA Corporation of the United States; NanoDrop2000 spectrophotometer DL2000Marker, agarose, and nucleic acid dyes were purchased from Beijing Suo Laibao Technology Co., Ltd.; 2×TaqPCR Mas...

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Abstract

The invention provides a method for detecting fecundity of alpine Merino sheep based on INHA gene, which relates to the technical field of genetic engineering, and detects the base of the first exon of INHA gene of alpine Merino sheep at 206bp . When the base is T, the genotype is TT; when the base is A, the genotype is TA or AA; the fecundity of the genotype AA is greater than that of the genotype TA, and the fecundity of the genotype TA is greater than that of the genotype TT. The invention can judge the fecundity of the alpine merino sheep by detecting the base at 206bp of the first exon ofthe INHA gene of the alpine merino sheep.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a method for detecting the fecundity of Alpine Merino sheep based on the INHA gene. Background technique [0002] The new breed of alpine Merino sheep is the world's first new breed of wool and meat Merino sheep that can adapt to the alpine cold and drought ecological zone at an altitude of 2400-4070m and the wool fiber diameter is 19.1-21.5μm. Excellent germplasm resources are provided (Yue Yaojing et al., 2014). Under grazing conditions, the lambing rate of alpine merino adult ewes is 110% to 120%, the reproductive rate is low, and the breeding cost is high, which greatly limits the development of the fine-wool sheep industry. At present, one of the important development directions of fine-wool sheep at home and abroad is to concentrate on cultivating new breeds (lines) of fine-wool sheep with multiple births on the basis of keeping the fiber diameter of fine wool ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 杨博辉郭婷婷袁超刘建斌牛春娥孙晓萍岳耀敬冯瑞林陈来运
Owner LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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