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Pseudoginsenoside 12-keto-pf11 and its extraction method and its medicinal use

One-PF11, pseudo-ginsenoside technology, applied in pseudo-ginsenoside 12-ketone-PF11 and its extraction, application field in medicine, can solve the problems of short drug effect time, high price, long cycle, etc., and achieve remarkable curative effect Effect

Active Publication Date: 2021-02-12
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above-mentioned treatment has the following problems: short drug effect time, high price and long cycle, long-term use of corticosteroid hormones and immunosuppressants have side effects such as aggravating microvascular damage, corticosteroid symptoms and destroying the body's immune system
In addition to the above American ginseng root polysaccharide extract CVT-E002 In addition, there are few reports on the research of American ginseng in allergic diseases, and the research on the specific octilon-type saponins in American ginseng in allergic diseases is blank.

Method used

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  • Pseudoginsenoside 12-keto-pf11 and its extraction method and its medicinal use
  • Pseudoginsenoside 12-keto-pf11 and its extraction method and its medicinal use
  • Pseudoginsenoside 12-keto-pf11 and its extraction method and its medicinal use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Take 10kg of dried American ginseng flower buds, crush them, add 4 times the amount of 85% ethanol to heat and reflux for extraction, each time for 3 hours, and extract 4 times in total, combine the extracts, recover the solvent to dryness, suspend it with water, and add it to AB In -8 macroporous adsorption resin, rinse with water first, then elute with 40% ethanol, then elute with 75% ethanol, collect the 75% ethanol eluate, recover the solvent until dry, and then carry out 2 times of 200 mesh silica gel column Chromatography, eluent: chloroform: methanol: water = 10: 3; 1, the eluate was concentrated under reduced pressure and recovered to dryness, and carried out for C 18 Reverse-phase silica gel open column or medium-pressure preparative column separation, the eluent is acetonitrile: water = 40:60, recovered to dryness under reduced pressure, and recrystallized with ethyl acetate to obtain pseudoginsenoside 12-keto-PF11;

[0029] Structural identification by spectr...

Embodiment 2

[0042] Take 20kg of dried American ginseng flower buds, crush them, add 3 times the amount of 80 ethanol to heat and reflux for extraction, each time for 4 hours, and extract 5 times in total, combine the extracts, recover the solvent to dryness, suspend it with water, and add it to D- 101 macroporous adsorption resin, wash with water first, then add 30% ethanol for elution, then add 70% ethanol for elution, collect the 70% ethanol eluate, recover the solvent until dry, and then conduct 2 times of 200 mesh silica gel column layer Analysis, eluent: n-butanol: ethyl acetate: water = 5: 1; 3, the eluate was concentrated under reduced pressure and recovered to dryness, and carried out C 18 Reverse-phase silica gel open column or medium-pressure preparative column separation, the eluent is methanol: water = 25:75, recovered to dryness under reduced pressure, and crystallized with ethanol to obtain pseudoginsenoside 12-keto-PF11.

Embodiment 3

[0044] Take 5 kg of dried American ginseng flower buds, crush them, add 5 times the amount of 95% ethanol to heat and reflux for extraction, each time for 2 hours, and extract 3 times in total, combine the extracts, recover the solvent to dryness, suspend it with water, and add it to D In -101 macroporous adsorption resin, rinse with water first, then elute with 45% ethanol, then elute with 85% ethanol, collect the 85% ethanol eluate, recover the solvent until dry, and then carry out three times of 400 mesh silica gel column Chromatography, eluent: dichloromethane: ethanol: water = 20: 5; 5, the eluate was concentrated under reduced pressure and recovered to dryness, and carried out for C 18 Reverse-phase silica gel open column or medium-pressure preparative column separation, eluent is acetonitrile: water = 75:25, recovered to dryness under reduced pressure, and recrystallized with methanol to obtain pseudoginsenoside 12-keto-PF11.

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Abstract

The invention relates to pseudo-ginsenoside 12-ketone-PF11, an extraction method and its medical use, belonging to the field of medicine. Take an appropriate amount of American ginseng flower buds, add 3 to 5 times the amount of 80 to 95% ethanol and heat and reflux for extraction, each time for 2 to 4 hours, and extract 3 to 5 times in total. Silica gel column chromatography, C 18 Reverse-phase silica gel open column or medium-pressure preparative column separation, recrystallization in organic solvents such as methanol to obtain pseudoginsenoside 12-one-PF11. The invention has carried out animal model experiments, can reduce the death rate of anaphylactic shock in mice, reduce ear swelling in mice with delayed hypersensitivity, and inhibit the increase of capillary permeability in mice caused by 5-hydroxytryptamine and histamine. Pseudoginsenoside 12-ketone-PF11 of the present invention is expected to be developed as a new type of drug for the prevention and treatment of allergic diseases as a pharmaceutical active ingredient, and provide clinical guidance and basis for the prevention and treatment of allergic diseases, thereby reducing the suffering of patients. Improve patient quality of life.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to pseudoginsenoside 12-keto-PF11 and its extraction method and its application in the preparation of medicines for treating allergic diseases. Background technique [0002] Allergic disease is a type I allergic disease caused by the body's exposure to external allergens, also known as immediate or allergic allergic disease. Clinically, allergic diseases include urticaria, eczema, characteristic dermatitis, allergic asthma, allergic rhinitis and other diseases. With social development, accelerated industrialization and changes in people's lifestyles, the prevalence of allergic diseases continues to increase, and allergic diseases seriously affect the quality of life of patients. At present, the commonly used drugs for clinical treatment of allergic diseases are antihistamines, corticosteroids and immunosuppressants. However, the above treatment has the following problems: short...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J17/00A61P37/00
CPCA61P37/00C07J17/005
Inventor 祁增李平亚王振洲周柏松王翠竹王涵吴福林董庆海林红强刘金平
Owner JILIN UNIV
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