Microsatellite marker primers for individual identification of sterlet and method for individual identification

Abstract

The invention discloses a microsatellite marker primer for individual identification of corpus sturgeon, comprising 10 pairs of microsatellite marker primers for corpus sturgeon, the nucleotide sequence of the 10 pairs of marker primers is SEQ ID NO: 1-20; the present invention Also disclosed is a method for individual identification of sterlets, comprising the following steps: step 1: taking different sterlet tissue samples respectively, and then extracting DNA from the sterlet tissue samples; step 2: using the microsatellite described in step 1 Carry out PCR amplification on the DNA of the sterlet tissue sample with the labeled primers to obtain PCR amplification products; step 3: electrophoresis separate the PCR amplification products on polyacrylamide gel, and then count the PCR amplification products according to the electrophoretic separation results Genotype; step 4: Carry out individual identification to sterlet according to the genotype of the PCR amplification product; use the method of the present invention to effectively detect genetic diversity and individual identification to sterlet.

Description

technical field [0001] The invention belongs to the technical field of biological individual identification, in particular to a microsatellite marker primer for individual identification of sterlet sturgeon, and also relates to a method for individual identification using the microsatellite marker primer. Background technique [0002] The sterlet is a fish of the genus Acipenseridae, mainly distributed in fresh water in Europe. The body is long and conical, and the body color changes greatly. The back is often dark gray-brown, and the abdomen is yellow-white. Omnivorous, small fish and shrimp, aquatic plants. There are migratory species and sedentary species, and they feed on benthic organisms in rivers, mainly aquatic insect larvae, crustaceans and small molluscs. During the fish spawning period, sterlets feed on the eggs of other sturgeons and other fish, and sometimes fish eggs account for more than 50% of their food intake. The sterlet hardly feeds in winter when the ...

AI Technical Summary

Problems solved by technology

[0003] The appearance of the scorpion is familiar, and it is difficult to identify the scorpion from the appearance. Generally, the way to identify the scorpion in production is to mark the scorpion. However, this method has a disadvantage. When the scorpion The markings on the body of the sterlet are washed away by the water, so it is difficult to distinguish the individual sterlet

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