Anti-Ebola Virus Activity Screening Method of Small Molecule Synthesized Compound 1712

Abstract

The invention discloses a small-molecule synthetic compound 1712 anti-ebola virus activity screening method, and relates to the technical field of antiviral drugs. The small-molecule synthetic compound 1712 anti-ebola virus activity screening method includes the step: culturing human embryonic kidney cells (293T), human pulmonary epithelial cells (A549) and HeLa cells. A complete screening systemis formed by cell culture, pseudovirus preparation, small-molecule compound preparation, anti-EBOV pseudovirus activity screening, preliminary action mechanism research, infectious EBOV experiments and experimental result analysis, all links of the screening system relate to specific experimental steps and comparative verification of experimental results, the results are high in reliability, related operation steps are described in all the links, and scientificity of the screening mode and method is further embodied.

Description

technical field [0001] The invention relates to the technical field of antiviral drugs, in particular to a method for screening the anti-ebola virus activity of the small molecule synthetic compound 1712. Background technique [0002] Ebola virus is transmitted through bodily fluids. Mucus, saliva or blood are all mediums. It can even be transmitted by shaking hands. Ebola is a deadly bloody fever infection that can infect humans, monkeys and chimpanzees. In primates, more than half of human patients bleed to death within two weeks. [0003] At present, there have been many literature reports on the study of small molecule compounds in different cycles of EBOV replication infection, and the molecular types are various, such as the compound LJ001 that inhibits the early stage of EBOV replication infection, and the cathepsin CatB / L inhibitor, all of which belong to amide derivative compounds , Shah et al. reported in 2010 that using a compound called tetrahydroquinoline oxidi...

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies of the prior art, the present invention provides a screening method for the anti-ebola virus activity of the small molecule synthetic compound 1712, which solves the problem that the authority of the literature report still needs to be verified through rigorous experiments, but the small molecule synthesis The screening method and application method of compound 1712 against ebola virus did not have any problems in specific reports

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