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Enzyme immunodetection lath, enzyme immunodetection board and detecting method thereof

A technique of enzyme immunoassay and slats, applied in the field of enzyme immunoassay

Pending Publication Date: 2019-04-23
SHANGHAI WEIYIN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] One of the objectives of the present invention is to provide an enzyme immunoassay strip, an enzyme immunoassay board and a detection method thereof, so as to solve the problem that the microporous plate in the prior art cannot realize multiple test items for one sample at the same time in one microporous plate Questions that can be detected in any combination

Method used

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  • Enzyme immunodetection lath, enzyme immunodetection board and detecting method thereof
  • Enzyme immunodetection lath, enzyme immunodetection board and detecting method thereof
  • Enzyme immunodetection lath, enzyme immunodetection board and detecting method thereof

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Embodiment 1

[0052] figure 1 It is a schematic diagram of the assembly structure of a microporous strip body 10 and an annular body 11 provided in Embodiment 1 of the present invention, figure 2 It is a schematic structural view of a microporous strip body 10 provided in Embodiment 1 of the present invention, image 3 It is a schematic structural diagram of a ring body 11 provided in Embodiment 1 of the present invention, Figure 4 It is a schematic structural diagram of a ring body 11 provided in Embodiment 1 of the present invention, Figure 5 It is a schematic structural diagram of an enzyme immunoassay panel provided in Example 1 of the present invention, Figure 6 yes Figure 5 Partial enlarged schematic diagram at A, please refer to Figure 1-6 , an enzyme immunoassay strip, comprising a microporous strip body 10 and a ring body 11, one end of the microporous strip body 10 is recessed inward for the first time to form a first groove, and the first concave The groove has a first...

Embodiment 2

[0073] The present invention also provides an enzyme immunoassay board, comprising a plurality of the above enzyme immunoassay board strips and an outer frame support frame 18, each of the enzyme immunoassay board strips is detachably connected to the outer frame support frame 18 . Realize the free combination of solid-phase carriers according to the specific conditions of patients in clinical practice to complete the detection of multiple test items of the same specimen that can be combined arbitrarily, and meet the requirements of highly individualized clinical detection. The outer wall of the ring body 110 and the micropore 12 There is an annular gap between the side walls, the annular body 110 includes a hollow channel for communicating with the bottom of the microhole 12 and the outside world, there is a gap between the end surface of the annular body 110 near the bottom of the microhole 12 and the microhole 12 The communication channel used to connect the hollow channel ...

Embodiment 3

[0079] The present invention also provides a detection method of enzyme immunity, adopts above-mentioned enzyme immunity detection plate, comprises the following steps:

[0080] S1: Coating a set antigen or set antibody on the ring body 11;

[0081] S2: connecting the annular body 11 to the microporous strip body 10;

[0082] S3: Add the mixture of the sample and the enzyme conjugate into the enzyme immunoassay strip, and the liquid surface of the mixture of the sample and the enzyme conjugate is submerged in the annular body 110 and located at the Below the first plane mentioned above, set the incubation time;

[0083] S4: Pour off the liquid in the enzyme immunoassay strip after the treatment in S3, and wash it;

[0084] S5: After cleaning, add the chromogenic agent into the enzyme immunoassay strip, after shaking, the liquid level of the chromogenic agent is below the end surface of the other end of the annular body 110, and after incubation for a set time Analyze test r...

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Abstract

The invention provides an enzyme immunodetection lath, an enzyme immunodetection board and a detecting method thereof. The enzyme immunodetection lath comprises a micropore lath body and circular bodies, each circular body comprises a circular main body and a connection piece, each circular main body is at least partially located in a micropore, a circular gap exists between each circular main body and each micropore, each circular gap is communicated with a first groove, one end of each connection piece is connected to the part, close to a first plane, of the circular main body, and the otherend of the connection piece is detachably connected with the micropore lath body. Each circular main body comprises a hollow channel used for connecting the bottom of the micropore and the outside, and a communication channel used for communicating the hollow channel and the circular gap exists between one end face, close to the bottom of the micropore, of the circular main body and the micropore. The enzyme immunodetection lath provided by the invention clinically achieves free combination of a solid-phase carrier according to the specific situation of a patient so as to achieve detection ofany combinations of multiple test projects of a same sample, improves the enzyme immunodetection benefits, and has the advantages of high sensitiveness, good repeatability and short detection time.

Description

technical field [0001] The invention belongs to the technical field of enzyme immunoassay, and relates to an enzyme immunoassay strip, an enzyme immunoassay board and a detection method thereof. Background technique [0002] Ordinary detachable 96-well microplate is composed of multiple microwell strips fixed on the frame. A microwell strip is usually composed of 8-12 cup-shaped microwells. The disassembled microwells can be fixed to It is used for detection on the bracket. A microwell integrates multiple functions. It is not only a solid phase carrier for antigen antibody protein, but also an immunobiochemical reactor. Since the bottom of the microwell is transparent, the interpretation of the instrument can be completed by visual inspection, but if a sample It is necessary to carry out the detection of any combination of multiple test items at the same time, such as the detection of multi-item allergen sIgE, then it is necessary to disassemble and reassemble the microporou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/58G01N33/543
CPCG01N33/54306G01N33/581
Inventor 贺坚慧
Owner SHANGHAI WEIYIN BIOTECH CO LTD
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