Full-human huTIGIT monoclonal antibody and application thereof

A monoclonal antibody and fully human technology, applied in the field of genetic engineering products, can solve problems such as application limitations

Active Publication Date: 2019-05-10
ANHUI ANKE BIOTECHNOLOGY (GRP) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, due to the production of human anti-mou...

Method used

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  • Full-human huTIGIT monoclonal antibody and application thereof
  • Full-human huTIGIT monoclonal antibody and application thereof
  • Full-human huTIGIT monoclonal antibody and application thereof

Examples

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Embodiment Construction

[0033] specific implementation plan

[0034] The following examples only further illustrate the present invention, and should not be construed as limiting the present invention. The Examples do not include detailed descriptions of conventional methods, such as those used to construct vectors and plasmids, to insert genes encoding proteins into such vectors and plasmids, or to introduce plasmids into host cells. Such methods are well known to those of ordinary skill in the art.

[0035] Antibody library construction

[0036] In this study, the phage display vector pComb3 was modified to obtain the phage display vector pC3XFSS suitable for the library construction system of our laboratory. Refer to the library construction method in related books (Therapeutic Antibody Methods and Protocols, Antony S.Dimitrov, 2009; Phage Display in Biotechnology and Drug Discovery, Sachdev S.Sidhu, 2005.), using QIAEX II Gel Extraction Kit from Qiagen, Axygen The PCR cleaning recovery kit, DN...

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Abstract

The invention relates to an anti-human TIGIT monoclonal antibody which is particularly related to high-affinity anti-human TIGIT monoclonal antibody with blocking activity. A large-capacity fully-synthetic human phage antibody library is constructed, the specific anti-human TIGIT monoclonal antibody and the high-affinity anti-human TIGIT monoclonal antibody which is optimized after molecular evolution are screened from the human phage antibody library, and the monoclonal antibody comprises a full-human frame region and variable regions of a full-human light chain and a heavy chain.

Description

technical field [0001] The present invention relates to genetic engineering products, that is, several fully human anti-huTIGIT specific monoclonal antibodies with high affinity and blocking activity are disclosed. The present invention constructs a large-capacity fully synthetic human phage antibody library, from which four preferred fully human anti-human TIGIT antibodies TBB8, TDC8, 3TB3 and 5TB10 were screened and optimized. The preferred antibody D1Y1A of the present invention has higher affinity and blocking activity, and can effectively recognize human TIGIT antigen. Background technique [0002] In recent years, therapeutic antibodies have shown increasingly broad application prospects. However, due to the production of human anti-mouse antibody (HAMA), its application is greatly limited. The emergence of antibody library technology provides a new way to solve this problem, and provides an effective way to obtain antibodies to various immunogens, including self-ant...

Claims

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Application Information

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IPC IPC(8): C07K16/18A61K39/395A61P35/00G01N33/574G01N33/577
Inventor 鲍文英刘浩叶洪涛周伟李道远范清林宋礼华
Owner ANHUI ANKE BIOTECHNOLOGY (GRP) CO LTD
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