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Traditional Chinese medicine injection for reversing drug resistance of staphylococcus aureus

A staphylococcus, drug-resistant technology, applied in the direction of antibacterial drugs, pharmaceutical formulations, organic active ingredients, etc., can solve the problems of no drug available for clinical infection treatment, reduced sensitivity of vancomycin, etc., to achieve the elimination of drug-resistant plasmids, The effect of restoring sensitivity and reversing drug resistance

Active Publication Date: 2019-06-25
SHANGHAI KAIBAO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The clinical treatment of MRSA infection mainly relies on vancomycin, but in 1997, it was discovered in Japan that the clinical isolation of MRSA showed a phenomenon of reduced sensitivity to vancomycin. In 2002, the clinical isolation of vancomycin-resistant Staphylococcus aureus ( VRSA), once this bacterium is prevalent, there will be basically no drugs available for clinical infection treatment

Method used

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  • Traditional Chinese medicine injection for reversing drug resistance of staphylococcus aureus
  • Traditional Chinese medicine injection for reversing drug resistance of staphylococcus aureus
  • Traditional Chinese medicine injection for reversing drug resistance of staphylococcus aureus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Embodiment 1, the mensuration of clinical separation MRSA minimum inhibitory concentration value

[0066] Strains to be tested: strain 161017, strain 1611099 and strain ATCC43300.

[0067] 1. Activation and cultivation of bacteria

[0068] Inoculate a single colony of the strain to be tested in MH liquid medium, culture with shaking at 37°C and 280rpm for 18-24h, collect the bacterial liquid, and dilute to OD with MH liquid medium 600nm =0.02, the bacteria solution to be tested was obtained.

[0069] 2. Determination of minimum inhibitory concentration

[0070] 1. Take the diluted Tanreqing injection, use MH liquid medium according to different volume ratios (1 / 2, 1 / 4, 1 / 8, 1 / 16, 1 / 32, 1 / 64, 1 / 128, 1 / 256, 1 / 512, 1 / 1024, Tanreqing injection is 1 part by volume) to dilute to obtain Tanreqing test solution.

[0071] 2. Use MH liquid medium to prepare vancomycin at different concentrations (8 μg / mL, 4 μg / mL, 2 μg / mL, 1 μg / mL, 0.5 μg / mL, 0.25 μg / mL, 0.125 μg / mL, 0.063 μ...

Embodiment 2

[0088] Example 2. Induction of Tanreqing Injection to Clinically Isolated Methicillin-resistant Staphylococcus aureus

[0089] 1. Induction of strain 161017 by Tanreqing injection

[0090] 1. Take 1 part by volume of diluted Tanreqing injection, and dilute it to 8 parts by volume with MH liquid medium to obtain MH liquid medium (medium A) containing Tanreqing. Inoculate strain 161017 in culture medium A, shake culture at 37°C and 280rpm for 16-18h, take 0.1mL of bacterial liquid and spread evenly on MH solid medium, and culture at 37°C until the colony grows, which is recorded as reversal 1 generation .

[0091] Inoculate the strain 161017 in the MH liquid medium, shake and culture at 37°C and 280rpm for 16-18h, take 0.1mL of the bacterial liquid and evenly spread it on the MH solid medium, and culture it statically at 37°C until the colony grows, which is recorded as the first generation of reversal control.

[0092] 2. Inoculate the reversed generation 1 single colony obt...

Embodiment 3

[0122] Embodiment 3, the minimum inhibitory concentration value determination of each generation bacterial strain after induction

[0123] Strains to be tested: strain 161017 (primary generation, reversal 1 generation - reversal 5 generation, reversal 1 generation control - reversal 5 generation control), strain 1611099 (primary generation, reversal 1 generation - reversal 5 generation, reversal 1 generation control - reversal 5 generation control) and strain ATCC43300 (primary generation, reversal 1 generation-reversal 5 generation, reversal 1 generation control-reversal 5 generation control).

[0124] 1. Activation and cultivation of bacteria

[0125] Inoculate a single colony of the strain to be tested in MH liquid medium, culture with shaking at 37°C and 280rpm for 18-24h, collect the bacterial liquid, and dilute to OD with MH liquid medium 600nm =0.02, the bacteria solution to be tested was obtained.

[0126] 2. Determination of minimum inhibitory concentration

[0127...

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Abstract

The invention discloses a traditional Chinese medicine injection for reversing drug resistance of staphylococcus aureus. The invention provides an application of Tanreqing in preparation of products;the use of the product comprises at least one of the following uses (a1)-(a3): (a1) reduction of the drug resistance of drug-resistant bacteria; (a2) treatment of drug-resistant bacteria infectious diseases; and (a3) inhibition of spreading of the drug-resistant bacteria. The Tanreqing injection liquid is composed of five traditional Chinese medicines: scutellaria baicalensis, bear gall powder, cornu gorais, honeysuckle and fructus forsythiae. By changing a part of phenotypic characteristics of the bacteria, drug-resistant plasmids are eliminated, the sensitivity degree of the bacteria to antibacterial agents is restored, the drug resistance of the drug-resistant bacteria to different kinds of antibiotics can be effectively reversed, and great significance on treating drug-resistant bacteria infectious diseases and inhibiting the spreading of the drug-resistant bacteria is achieved.

Description

technical field [0001] The invention relates to a traditional Chinese medicine injection for reversing drug resistance of Staphylococcus aureus. Background technique [0002] Due to the irrational use and abuse of antibacterial drugs, bacteria are exposed to the selection pressure of antibacterial drugs for a long time, resulting in the emergence of a large number of drug-resistant bacteria. At present, bacterial drug resistance has become a major problem that seriously endangers human health, and it has also become a huge challenge facing the world. The development of new antibiotics against drug-resistant bacteria is undoubtedly the main way and method to solve the current infection of drug-resistant bacteria. It generally takes about 10 years for researchers to develop a new antibiotic from discovery to identification and then to large-scale production, but it only takes 2 years for the emergence of drug-resistant bacteria. The speed of antibiotic development is far behi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/14A61K36/634A61P31/04A61K31/7036
CPCY02A50/30
Inventor 王毅杨伟峰李连达孙娅楠姜童童
Owner SHANGHAI KAIBAO PHARMA
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