Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of composition containing anti-CD45 monoclonal antibody and using method thereof

A composition and antibody technology, applied in the field of molecular biology, can solve the problems of large amount of antibodies, low affinity of white blood cells, and high cost

Active Publication Date: 2022-07-05
SHANGHAI BAIZE MEDICAL LAB CO LTD +2
View PDF12 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the use of different combinations of antibodies against leukocyte subtypes, the amount of antibodies required is larger and the cost will be higher
The antibodies used by MINDEC are mouse monoclonal antibodies, and the antibodies extracted from mice have low affinity to human leukocytes, so there is a great demand for mouse monoclonal antibodies in the MINDEC method

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of composition containing anti-CD45 monoclonal antibody and using method thereof
  • A kind of composition containing anti-CD45 monoclonal antibody and using method thereof
  • A kind of composition containing anti-CD45 monoclonal antibody and using method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0144] The blood of the 3 patients was divided into 3 tubes, each tube was 2ml, and the total number of residual white blood cells and the number of CTCs were detected by using the mass ratio of two CD45 antibodies with high affinity and low affinity of 1:1, 5:1, and 10:1 respectively. Compare.

[0145] The result is as figure 1 shown. The results showed that when the mass ratio of high-affinity and low-affinity CD45 antibodies was 1:1, 5:1, and 10:1, the total number of residual leukocytes decreased in turn, indicating that the ability to remove leukocytes gradually increased.

[0146] The total number of detected CTCs in each sample is counted, and the results are as follows figure 2 shown. The results show that the mass ratio of 5:1 is better than 1:1 and 10:1. The possible reason is that the amount of high-affinity antibody in the dosage of 1:1 is too low, and there are too many leukocytes remaining, which leads to the missed detection of CTC cells; while in the dosag...

Embodiment 2

[0148] Two tubes of equal volume of peripheral blood were taken from the same tumor patient, and the experimental operation was repeated three times, that is, two tubes of peripheral blood were collected from 15 patients with different tumors. CTC detection was carried out according to the method described in the control (Scitech Bio; US Patent No.: US8,969,021) and the method described in the "Materials and Methods" section of this paper, and the final detection rate was evaluated. The mass ratio of the two CD45 antibodies was 5:1. The number of CTC detected by the two detection methods is as follows: image 3 shown.

[0149] The total number of cells in each sample was scanned and counted, and the results were as follows Figure 4 shown. The results show that the total number of residual blood-derived leukocytes of the present invention is lower than that of the control, indicating that the present invention has the ability to efficiently enrich CTCs.

[0150] Count and ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
affinityaaaaaaaaaa
recovery rateaaaaaaaaaa
Login to View More

Abstract

The present invention provides a method and application for enriching and identifying circulating tumor cells based on two or more high-affinity anti-CD45 rabbit monoclonal antibodies. Specifically, the present invention provides a composition containing two or more CD45 rabbit monoclonal antibodies, which can recognize different antigenic determinants on the CD45 antigen molecule. The present invention also includes methods of using the composition in combination with anti-CD16 mAb, anti-CD19 mAb and anti-CD235a mAb to isolate leukocytes, enrich and identify circulating tumor cells. The antibody combination of the present invention is a rabbit monoclonal antibody with high affinity and strong specificity, which can better label various leukocyte subtypes, thereby better removing leukocytes, and more efficiently enriching and identifying CTCs without damaging CTCs. cells, so that CTC cells maintain a good natural state and cell morphology.

Description

technical field [0001] The invention belongs to the field of molecular biology, and relates to a method for enriching and identifying circulating tumor cells based on two or more high-affinity anti-CD45 rabbit monoclonal antibodies and its application. Background technique [0002] Circulating tumor cells (Circulating Tumor Cells, CTCs) refer to solid tumor epithelial cells that shed from the primary tumor and enter the peripheral blood circulation system during tumor growth. As the link between the primary tumor and the metastatic tumor, CTCs will attach to the distal tissue through the blood circulatory system and grow to form new tumor tissue, that is, the metastatic tumor. A large number of clinical data show that tumor metastasis is the direct cause of death of tumor patients. Therefore, the detection of the number of CTCs can realize real-time monitoring of tumor development. It has important clinical research value in guiding clinical treatment, evaluating treatment ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/536G01N33/543
CPCG01N33/536G01N33/54326G01N33/543G01N33/577
Inventor 钱其军叶真龙马硕王欣玥张晓霞
Owner SHANGHAI BAIZE MEDICAL LAB CO LTD