Method for extracting nucleic acid and extraction cassette thereof

An extraction and cassette technology, applied in the field of extraction cassettes, can solve the problems of poor extraction efficiency and purity of magnetic beads, and achieve the effects of cost saving, pollution avoidance and volume reduction

Pending Publication Date: 2019-07-12
DELTA ELECTRONICS INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] There is also an all-in-one nucleic acid extraction module in the prior art, which uses magnetic bead extraction to extract nucleic acid, but the extraction efficiency and purity of magnetic beads are not good

Method used

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  • Method for extracting nucleic acid and extraction cassette thereof
  • Method for extracting nucleic acid and extraction cassette thereof
  • Method for extracting nucleic acid and extraction cassette thereof

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Experimental program
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Embodiment Construction

[0065] Figure 1A A combined diagram of the extraction cartridge C of the embodiment of the present invention is shown. Figure 1B An exploded view of extraction cartridge C of an embodiment of the present invention is shown. Please refer to Figure 1A , Figure 1B, the extraction cartridge C includes an extraction module 1 , a liquid storage module 2 , a sampling module 3 , a communication module 4 and other components. The extraction cartridge C is suitable for placement in an analyzer. The analyzer includes a first air pressure supply module, a second air pressure supply module, and a detection module, and the first air pressure supply module and the second air pressure supply module provide air pressure to the extraction cassette C to control the extraction cassette C liquid flow inside. The above detection module heats and dissipates heat from the extraction cassette C, and detects and analyzes the samples in the extraction cassette C.

[0066] Please refer to Figur...

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Abstract

The present invention provides an extraction cassette, which includes an extraction module. The extraction module includes an extraction module body, an expansion compartment, a reaction compartment,a filter, a collection compartment, a first waste-liquid compartment and a second waste-liquid compartment. The expansion compartment is formed on the extraction module body. The reaction compartmentincludes a reaction compartment inlet, a reaction compartment outlet and a reaction compartment notch, the expansion compartment is connected to the reaction compartment notch, and the reaction compartment notch is located between the reaction compartment inlet and the reaction compartment outlet. The filter is disposed in the reaction compartment and corresponding to the reaction compartment outlet. The collection compartment communicates with the reaction compartment outlet. The first waste-liquid compartment communicates with the reaction compartment outlet. The second waste-liquid compartment communicates with the reaction compartment outlet.

Description

technical field [0001] Embodiments of the present invention relate to an extraction cassette, in particular to an extraction cassette with a liquid storage module. Background technique [0002] The nucleic acid extraction module in the prior art mainly only provides the function of nucleic acid extraction, and does not have a waste liquid chamber design for accommodating waste liquid. Since the waste liquid may flow back and contaminate the filter membrane in the reaction chamber, in the prior art, the waste liquid chamber is not arranged adjacent to the collection chamber. Since the waste liquid chamber and the reaction chamber need to be arranged under different module kits, the size of the extraction cassette becomes very large, which not only takes up space, but also increases the cost. [0003] There is also an all-in-one nucleic acid extraction module in the prior art, which utilizes magnetic bead extraction to extract nucleic acid, but the extraction efficiency and p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10
CPCC12N15/10B01D11/04
Inventor 锺崴宇黄菘斌刘兴伦高于凯李怡臻
Owner DELTA ELECTRONICS INC
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