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A device and method for acclimating and cultivating methane-oxidizing bacteria simulating underground coal mine environment

A technology for methane-oxidizing bacteria and domestication and cultivation is applied in the field of domestication and cultivation devices for methane-oxidizing bacteria, which can solve the problems of inability to effectively simulate the pH and atmospheric environment of coal seams.

Active Publication Date: 2022-04-08
SHANDONG UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

However, the existing experimental device for methane-oxidizing bacteria to degrade raw coal adsorbed gas cannot effectively simulate the pH and atmospheric environment in coal seams.

Method used

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  • A device and method for acclimating and cultivating methane-oxidizing bacteria simulating underground coal mine environment
  • A device and method for acclimating and cultivating methane-oxidizing bacteria simulating underground coal mine environment

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Experimental program
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Embodiment Construction

[0040] The present invention will be described in detail below in combination with specific embodiments.

[0041] The inventive device such as figure 1 As shown, it includes raw coal stress loading part (computer 1, pressure sensor 15, pressure monitor 16, pressure controller 22, oil pressure pump 23), environment temperature control part (water bath heating device 13, water bath temperature monitor 14), environment Gas atmosphere injection part (inlet 6, inlet valve 7, valve 8, hydrogen sulfide gas 9, carbon monoxide gas 10, carbon dioxide gas 11), methane gas injection part (inlet 6, inlet valve 7, high pressure Methane gas 12, valve 24), methane oxidizing bacteria liquid injection part (methan oxidizing bacteria liquid 19, liquid injection valve 20, liquid injection pipeline 21), methane gas online monitoring part (computer 1, CH 4 Sensor 17, CH 4 Gas monitor 18), environmental gas chromatographic analysis part (computer 1, gas chromatograph 4, gas collection port 5), env...

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Abstract

The invention discloses a device and method for acclimating and cultivating methane-oxidizing bacteria for simulating the underground environment of coal mines. Including raw coal stress loading part, ambient temperature control part, ambient gas atmosphere injection part, methane gas injection part, methane oxidizing bacteria liquid injection part, methane gas online monitoring part, environmental gas chromatography analysis part, environmental liquid phase mass spectrometry analysis part and environmental pH The control part consists of nine parts. The beneficial effect of the invention is that it can accurately and effectively simulate the microbial enrichment state of coal mine underground conditions, and simulate coal seam stress, temperature, pH and gas atmosphere conditions.

Description

technical field [0001] The invention belongs to the technical field of coal mining, and relates to a methane-oxidizing bacteria domestication and cultivation device and method for simulating the underground environment of coal mines. Background technique [0002] my country is a developing country, and energy is very scarce. Coal accounts for more than 70% of my country's total energy consumption. The output of coal directly affects the development of the national economy. Or high-gas mining areas are actively creating conditions for mining. And all coal mines in my country are gas mines. According to statistics, among large and medium-sized coal mines, high gas mines account for 20.34%, and outburst mines account for 19.77%. Among small coal mines, high gas mines account for about 15%. This is one of the reasons for more gas disasters. During the period from 2007 to 2016 alone, there were 389 various gas disaster accidents across the country, resulting in 3,307 deaths. A...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12M1/38C12M1/36C12M1/34C12M1/04C12N1/36
CPCC12M29/06C12M41/22C12M41/26C12M41/38C12M41/34C12M41/40C12M41/48C12N1/36
Inventor 谢军辛林程卫民杜海刚宋玥付海政王怡任广意
Owner SHANDONG UNIV OF SCI & TECH
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