Methane-oxidizing bacterium habituated culturing device capable of simulating coal mine downhole environment and method

Abstract

The invention discloses a methane-oxidizing bacterium habituated culturing device capable of simulating coal mine downhole environment and a method. The methane-oxidizing bacterium habituated culturing device comprises nine parts of a raw coal stress loading part, an environment temperature control part, an environment gas atmosphere injecting part, a methane gas injecting part, a methane-oxidizing bacterium fluid injecting part, a methane gas online monitoring part, an environment gas chromatographic analysis part, an environment liquid phase mass spectrometric analysis part and an environment pH control part. The methane-oxidizing bacterium habituated culturing device disclosed by the invention has the beneficial effects that the microorganism enrichment state under coal mine downhole condition can be accurately and effectively simulated, and the conditions of the coal seam stress, the temperature, the pH and the gas atmosphere are simulated.

Description

technical field [0001] The invention belongs to the technical field of coal mining, and relates to a methane-oxidizing bacteria domestication and cultivation device and method for simulating the underground environment of coal mines. Background technique [0002] my country is a developing country, and energy is very scarce. Coal accounts for more than 70% of my country's total energy consumption. The output of coal directly affects the development of the national economy. Or high-gas mining areas are actively creating conditions for mining. And all coal mines in my country are gas mines. According to statistics, among large and medium-sized coal mines, high gas mines account for 20.34%, and outburst mines account for 19.77%. Among small coal mines, high gas mines account for about 15%. This is one of the reasons for more gas disasters. During the period from 2007 to 2016 alone, there were 389 various gas disaster accidents across the country, resulting in 3,307 deaths. A...

AI Technical Summary

Problems solved by technology

However, the existing experimental device for methane-oxidizing bacteria to degrade raw coal adsorbed gas cannot effectively simulate the pH and atmospheric environment in coal seams.

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