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Uses of seabuckthorn endophytic fungus strain sj1 fermented extract

A technology of endophytic fungi and extracts, applied in the field of microbial applications, can solve problems such as pesticide residues and environmental pollution, and achieve the effects of improving resistance, economical use, and enhancing efficiency

Active Publication Date: 2021-01-05
SHANDONG AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the prevention and control of plant virus diseases mainly uses chemical drugs. Although it has played a certain role in the prevention and control of the occurrence and development of virus diseases, it has also brought problems such as environmental pollution and pesticide residues.

Method used

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  • Uses of seabuckthorn endophytic fungus strain sj1 fermented extract
  • Uses of seabuckthorn endophytic fungus strain sj1 fermented extract
  • Uses of seabuckthorn endophytic fungus strain sj1 fermented extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: 1. Preparation of seabuckthorn endophytic fungus strain SJ1 fermentation extract

[0023] The Paecilomyces variotii strain SJ1 isolated from seabuckthorn endophytic fungus was placed on a plate PDA medium, cultured at 25°C for 6 days, and inoculated into 50 mL of seed culture solution (for 250mL Erlenmeyer flask that PDA culture medium is not made with agar), at 28 ℃, cultivated on a rotary shaker at 120r / min for 3 days as seeds, inoculated with 10% amount into 150mL fermentation medium (fermentation medium is: potato Extract 1.0L, yeast extract 1.0g, peptone 3.0g, glucose 15.0g, agar 17.0g.Preparation of potato extract: take 200g of peeled potatoes, cut into small pieces, add 1000mL of distilled water and boil for 30 minutes, filter and remove the potato pieces , the filtrate was replenished to 1000mL) in the 500mL Erlenmeyer flask, cultivated under the same conditions for 5 days, and terminated the fermentation. The cultured mycelium was washed, dried at 6...

Embodiment 2

[0024] Example 2: Seabuckthorn endophytic fungus strain SJ1 fermented extract induces accumulation of salicylic acid

[0025] Stir the nutritious soil containing vermiculite and substrate (volume ratio 1:2) evenly, put it into a disposable plastic small bowl; sprinkle 3-4 seeds of Bunsen tobacco in each small bowl, and put it in the greenhouse (24°C, 14h light, 10h dark), covered with a layer of plastic film culture. After emergence, the seedlings were transplanted into small plastic pots, one plant per pot, and placed in a greenhouse for management. When the tobacco grew to 4 weeks, 10 plants of Tobacco benthamiana were selected and sprayed with 150 ng / mL SJ1 fermentation extract and deionized water (CK) respectively, 10 mL per plant, and each treatment was repeated 3 times. After 2 hours, the leaves of N. bentheniana sprayed with water and SJ1 fermented extract were taken, and the content of salicylic acid (SA) was detected by liquid chromatography.

[0026] (1) Extraction...

Embodiment 3

[0035] Example 3: Seabuckthorn endophytic fungus strain SJ1 fermentation extract enhances plant RNA silencing efficiency

[0036] Select 10 plants of 4-week-old Tobacco benthamiana, divide them into two groups, spray 150 ng / mL SJ1 fermentation extract and deionized water, 10 mL per plant, and repeat 3 times for each treatment. After 2 hours, the Agrobacterium liquid containing pBI121-GFP was transiently infected (OD600=1.0), observed under long-wave ultraviolet light and photographed to record the change of fluorescence intensity, and the observation continued for 7 days. The result is as image 3 with Figure 4 shown.

[0037] Depend on image 3 It can be seen that the GFP fluorescence intensity in the infected area treated with SJ1 fermentation extract was the strongest on the 3rd day after infection, but it was significantly weaker than that in the infected area treated with deionized water. Collect 3dpi (3 days after inoculation) the GFP-infected area of ​​15 strains of ...

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Abstract

The invention discloses the use of the seabuckthorn endophytic fungus strain SJ1 fermented extract, including inducing the accumulation of endogenous salicylic acid in plants; enhancing the efficiency of plant RNA silencing; and improving the resistance of plants to viruses. The fermented extract of seabuckthorn endophytic fungus strain SJ1 provided by the present invention has a good preventive effect at concentrations of 150ng / mL and 100ng / mL, and the average preventive effects are respectively: 77.45% and 72.88%, both of which are 20% higher than the control guanidine. The preventive effect of copper acetate 5ug / ml (71.98%); comprehensive analysis, the use concentration cost of SJ1 fermented extract 100~150ng / mL is lower than 20% morphine. Copper acetate, the SJ1 fermented extract provided by the present invention is more economical to use.

Description

technical field [0001] The invention belongs to the technical field of microorganism application, in particular to the application of seabuckthorn endophytic fungus strain SJ1 fermented extract. Background technique [0002] Virus disease is one of the three major diseases of plants, which is common and serious, and its prevention and control is relatively difficult, commonly known as "cancer" of plants. At present, the prevention and control of plant virus diseases mainly uses chemical drugs. Although it has played a certain role in the prevention and control of the occurrence and development of virus diseases, it has also brought problems such as environmental pollution and pesticide residues. Biological antiviral agent is a new kind of biological pesticide, which has the characteristics of non-toxic, non-polluting, no residue, no killing of natural enemies, and protection of human and animal safety; it also has the advantages of less dosage, long-lasting effect and low co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/30A01P21/00A01P1/00
CPCA01N63/30
Inventor 朱常香彭春娥王庆彬王洪凤耿全政孔波张爱苓
Owner SHANDONG AGRICULTURAL UNIVERSITY
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