A nucleic acid delivery carrier and its preparation method and application

A delivery carrier and nucleic acid technology, which is applied in the field of nanomaterials, can solve the problems of oligopolyethyleneimine that does not have gene delivery, achieve high nucleic acid delivery efficiency, improve nucleic acid compression ability, and simple methods
CN107556493BActive Publication Date: 2020-07-31THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
3 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
Publication Date
2020-07-31

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention provides a nucleic acid delivery carrier, a preparation method and application of the nucleic acid delivery carrier. The nucleic acid delivery carrier is a nanometer self-assembled bodyof an amphiphilic fluorinated material; and the amphiphilic fluorinated material is perfluorooctane-substituted oligomeric polyethyleneimine. According to the nucleic acid delivery carrier provided bythe invention, oligomeric polyethyleneimine and the perfluorooctane material are bonded together, and due to strong hydrophobicity of the perfluor material, not only can the nucleic acid compressingcapability of oligomeric polyethyleneimine be improved, but also the efficiency of cell endocytosis and endosome escape can be improved; positive charges of oligomeric polyethyleneimine not only can be used for electrostatic composition of siRNA, but also can utilize proton sponge effect to promote the endosome escape in the system, and by the combination of electrostatic composition of siRNA andthe endosome escape, the nucleic acid delivery carrier not only has very-high silence efficiency under the serum-free condition, but also can show very high nucleic acid delivery efficiency in the presence of serum, and is expected to be applied in gene therapy based on siRNA or DNA.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the technical field of nanomaterials, and relates to a nucleic acid delivery carrier and its preparation method and application, in particular to a fluorinated material-based nucleic acid delivery carrier and its preparation method and application. Background technique

[0002] At present, gene therapy has been considered as a powerful means to treat many diseases, including tumors, viral infections and hyperlipidemia. However, due to the negative charge of nucleic acid itself and its large size, it is difficult for them to enter cells by themselves to function; at the same time, free nucleic acid is easily degraded by nucleases in the body and loses its function. Therefore, it is very important to develop a carrier that can protect the nucleic acid from degradation in the in vivo environment and at the same time help the nucleic acid to cross the cell membrane.

[0003] The design and preparation of non-viral vectors is a ver...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More