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The method for the selection and breeding of tetraploid golden crown fish and the establishment method and application of its strain

A tetraploid and allotetraploid technology, which is applied in the field of establishment of tetraploid golden crown fish strains, can solve the problems that restrict the large-scale production of sterile triploid fish, and the tetraploid fish strains have few germplasm resources and are very poor. It is difficult to produce milky white semen and other problems, to achieve the effect of strong resistance to stress, beautiful body shape and simple method

Active Publication Date: 2020-07-10
HUNAN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are great differences between the two fishes in terms of classification, ploidy, body shape and other biological characteristics, especially the diploid carp F 2 It is difficult to produce milky white semen, most of which are watery semen, and it is difficult to obtain surviving hybrid offspring, which leads to great difficulties in distant hybridization between the two
[0005] In addition, sexually fertile tetraploid fish lines are core parents for large-scale production of 100% sterile triploid fish (often exhibiting significant heterosis in terms of growth rate, disease resistance, stress tolerance, and meat quality) Germplasm resources, although some tetraploid fish strains have been developed at present, there are still very few germplasm resources of tetraploid fish strains that are sexually fertile. mass production

Method used

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  • The method for the selection and breeding of tetraploid golden crown fish and the establishment method and application of its strain
  • The method for the selection and breeding of tetraploid golden crown fish and the establishment method and application of its strain
  • The method for the selection and breeding of tetraploid golden crown fish and the establishment method and application of its strain

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Effect test

Embodiment 1

[0038] A method for selecting and breeding tetraploid golden crown fish by distant hybridization of the present invention comprises the steps:

[0039] (1) Selection and cultivation of broodstock: From February 1st to February 10th of that year, fishing was carried out with nets, and female improved allotetraploid crucian carp with obvious sexual maturity characteristics, no disease, no injury, and good physical signs were selected. carp and male diploid carp F 2 The female and male parents of distant hybrids are cultivated in a special pond. During the cultivation period in the special pond, pay attention to maintaining good water quality and sufficient oxygen content in the water, and do a good job in feeding and management, especially about one month before the breeding season. Stimulate with running water to promote the maturation of broodstock gonads;

[0040] (2) Artificial induction of broodstock: From April 21st to May 10th of that year, when the water temperature was...

Embodiment 2

[0083] A method for establishing a tetraploid golden crown fish strain of the present invention comprises the steps of: selecting abdominally enlarged, soft female tetraploid golden crown fish and robust, The male tetraploid golden crown fish with obvious semen can be seen by squeezing the abdomen to induce labor. Two injections are used. The first injection is 12 μg / kg of luteinizing hormone releasing hormone analogues for the female parent at 4-5 pm, and 12 μg / kg for the parental injection. The injection of luteinizing hormone-releasing hormone analog 6μg / kg, the second injection after 6h interval, the female parent was injected with chorionic gonadotropin 24μg / kg, luteinizing hormone-releasing hormone analog 24μg / kg and the posterior pituitary The prime is 1g / kg, inject luteinizing hormone releasing hormone analogue 10 μ g / kg to male parent, concrete injection amount is carried out appropriate adjustment according to broodstock body size; Then put into the cement pond by mal...

Embodiment 3

[0085] Utilize above-mentioned tetraploid golden crown fish and diploid golden crucian carp to breed and obtain triploid fish, concrete method comprises the following steps:

[0086] During the breeding period, the female diploid carp with enlarged abdomen and softness was selected as the female parent, and one-shot injection was used to induce labor. The chorionic gonadotropin was 600 μg / kg, and the luteinizing hormone releasing hormone analogue was 6 μg / kg. The concentration of dioconone was 1 μg / kg; a robust, well-developed, slender male tetraploid golden crown fish was selected as the male parent, and the injection method was adopted. The concentration of chorionic gonadotropin was 300 μg / kg, and that of luteinizing hormone After the injection, the ratio of male to female was 1:2, and they were placed in a circular spawning pool with a diameter of 15m for induction of labor. Some mesh pieces and brown pieces were put in the pool, and the water temperature was 24°C. Observe...

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Abstract

The invention discloses a method for breeding a tetraploid golden crown fish through distant hybridization. The method comprises the following steps of taking female improved allotetraploid crucian-carp as the female parent and F2 male diploid carp-crucian as the male parent to perform artificial spawning and artificial dry fertilization in breeding season, placing obtained fertilized eggs into anincubation tank for incubation in flowing water, and after all fry are spawned and swimming bladders can be seen, detecting and screening the spawned fry through biological methods to obtain the tetraploid golden crown fish. The bred tetraploid golden crown fish has the advantages of being bisexually fertile, rapid in growth, beautiful in shape, unique in body color, strong in fertility and stress resistance and the like and can serve as a beautiful ornamental fish as well as provide a precious tetraploid fish resource. The invention also provides a method for establishing tetraploid golden crown fish lines and application of the tetraploid golden crown fish and accordingly is of significance to genetic breeding and biological evolution of fishes.

Description

technical field [0001] The invention belongs to the technical field of distant hybridization of fish, and in particular relates to a method for breeding tetraploid golden-crowned fish through distant hybridization, a method for establishing a strain of tetraploid golden-crowned fish, and an application thereof. Background technique [0002] Through long-term systematic research, the research team used the distant hybridization of female common red crucian carp and male Xiangjiang wild carp, in F 1 Carp carp F 1 , crucian carp F 1 There are some fertile female and male individuals in the population, and the fertile female and male crucian carp F 1 Carp F 2 , crucian carp F 2 There are very few fertile females and males in the group, and most of the semen is watery semen. Through thousands of crucian carp F 2 Screening was carried out in the group, and the few fertile male and female individuals obtained were self-crossed to obtain allotetraploid crucian carp. Using dipl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/027
CPCA01K67/0275A01K2227/40
Inventor 刘少军王余德张明贺谭慧芳曹柳罗凯坤覃钦博张纯陶敏王石胡方舟刘庆峰
Owner HUNAN NORMAL UNIVERSITY
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