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Rapid detection of Escherichia coli in thin film culture device

A technique for Escherichia coli, coliforms, applied in comparison with previous methods for Escherichia coli colonies,
[0006] Need to be used in the field of counting food and beverage samples that can solve problems such as expensive, complex

Pending Publication Date: 2019-11-19
3M INNOVATIVE PROPERTIES CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the above methods are generally relatively expensive, involve multiple steps, require incubation periods of about 24 hours to 48 hours to enumerate colonies, and / or require relatively complex instrumentation and / or relatively highly trained personnel

Method used

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  • Rapid detection of Escherichia coli in thin film culture device
  • Rapid detection of Escherichia coli in thin film culture device
  • Rapid detection of Escherichia coli in thin film culture device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1 to 13

[0114] Examples 1 to 13 Manufacture of microbiological testing articles.

[0115] Culture medium preparation :

[0116] Prepare the mixtures shown in Table 1 in separate containers. The liquid volume of the mixture was brought up to 1000 mL with deionized water.

[0117] Preparation of the first tablet :

[0118] The mixtures shown in Tables 2 and 3 were prepared by first adding guar gum to water, followed by adding the remaining components and heating to 80C to pasteurize the medium. After the heating step, the mixture was allowed to cool to ambient temperature and stored at 4°C until use.

[0119] The liquid mixture was warmed to ambient temperature and coated onto a polyester film, and the coated layer was dried as disclosed in Example 1 of US Patent 5,384,766. The dry spread of each individual medium applied to the individual membranes is approximately 0.2 g / 155 cm 2 to 0.3g / 155cm 2 . The coated sheets were cut into 10.2 cm x 7.6 cm rectangles.

[0120] The spa...

Embodiment 1

[0130] The device was prepared by adhering a first piece coated with medium 87 to a second piece prepared as described above.

Embodiment 2

[0132] The device was prepared by adhering a first piece coated with medium 83 to a second piece prepared as described above.

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Abstract

A device for differentially enumerating colonies of coliform and Escherichia coli microorganisms is provided. The device comprises a water-impermeable first sheet; a water-impermeable second sheet attached to the first sheet; a dry, rehydratable culture medium comprising a lactose-fermentation indicator system, a [beta]-D-glucuronidase indicator system that comprises a plurality of compounds thatenhance [beta]-glucuronidase enzyme activity in Escherichia coli, a redox indicator system and a first cold-water soluble gelling agent adhered to the first sheet, the culture medium disposed in a microbial growth zone; and a second cold-water-soluble gelling agent adhered to the second sheet. The microbial growth zone is disposed between the first sheet and the second sheet. Methods of using thedevice are also provided.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of U.S. Provisional Patent Application 62 / 480,633, filed April 3, 2017, the disclosure of which is incorporated herein by reference in its entirety. Background technique [0003] In food and beverage safety testing, the presence or absence of coliform bacteria is considered an important evidence of quality. In many countries around the world, the amount of coliform bacteria allowed in beverages and certain foods, such as dairy products, is regulated. Coliform bacteria include fecal coliforms such as Escherichia coli. The presence of fecal coliforms in food or water samples is used as a primary indicator of fecal contamination of food or water and the possible presence of other pathogenic microorganisms. [0004] Methods for detecting, identifying and / or enumerating microorganisms in a food sample generally vary according to the nature of the food and the types of microorganisms that ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/06C12Q1/10C12Q1/40C12M1/22
CPCC12Q1/06C12Q1/10C12Q1/40C12Q2304/24C12Q2304/46C12Q2334/52C12M29/04C12M33/02C12M41/36
Inventor 塞拉嘉·常德拉帕蒂阿莱克·J·蒂加登黑利·A·萨多里斯
Owner 3M INNOVATIVE PROPERTIES CO
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