A method for simultaneously detecting the content of multiple components in creatine powder

A determination method, creatine technology, applied in the field of analytical chemistry, can solve problems such as difficult separation, interference in determination of creatine monohydrate content, unsatisfactory separation, etc., and achieve good reproducibility, rapid separation, and optimized chromatographic conditions.

Active Publication Date: 2022-03-04
BY HEALTH CO LTD
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

The detection methods of GB 24154-2015 and QB / T 2834-2006 use methanol-water (40:60) as the mobile phase to detect the content of creatine monohydrate. Because creatine monohydrate and dicyandiamide have similar properties, it is difficult to separate them. Spectrum such as Figure 4 As shown, the separation is not ideal, thus causing interference to the determination of creatine monohydrate content, thus affecting the quality monitoring of creatine powder

Method used

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  • A method for simultaneously detecting the content of multiple components in creatine powder
  • A method for simultaneously detecting the content of multiple components in creatine powder
  • A method for simultaneously detecting the content of multiple components in creatine powder

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Embodiment 1

[0040] 1. Instruments and reagents

[0041] 1. Instrument

[0042] Agilent 1260 high performance liquid chromatograph (with UV detector), ultrasonic instrument, electronic balance

[0043] 2. Reagents

[0044] Ammonia water, primary water, sodium dihydrogen phosphate (chromatographically pure)

[0045] Creatinine reference substance: Shanghai Yuanye (batch number: YS0923JA14, purity: 98%)

[0046] Dicyandiamide reference substance: China Institute for Food and Drug Control (batch number: 100206-201103, purity: 100%)

[0047] Creatine monohydrate reference substance: Shanghai Yuanye (purity: 98%)

[0048] Creatine powder: provided by BY-HEALTH Co., Ltd.

[0049] 2. Method

[0050] 2.1 Chromatographic conditions:

[0051] Chromatographic column: Phenomenex Kinetex 5μm XB-C18 100A LC Column 250mm×4.6mm;

[0052] Mobile phase: 10mmol / L sodium dihydrogen phosphate solution (adjust the pH value to 10.5 with ammonia water);

[0053] Flow rate: 0.8mL / min;

[0054] Column tem...

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Abstract

The invention discloses a method for simultaneously detecting the content of multiple components in creatine powder, comprising: accurately weighing a sample, adding purified water, ultrasonically extracting, constant volume of purified water, shaking evenly, and passing through a water-phase filter membrane to obtain Creatine powder test sample; get creatine powder test sample, adopt HPLC to detect, the condition that described HPLC detects is: adopt C18 chromatographic column, with sodium dihydrogen phosphate solution, and adjust pH value to 8-11.5 with ammonia water The mobile phase is used for isocratic elution, and the contents of creatine monohydrate, creatinine, and dicyandiamide are obtained according to the HPLC detection results. The method of the invention can realize rapid separation of the three components of creatine monohydrate, creatinine and dicyandiamide by optimizing the chromatographic conditions, and has good reproducibility. The method of the present invention can eliminate the interference of the by-products dicyandiamide and creatinine in the creatine powder to creatine monohydrate, and can accurately detect the contents of creatine monohydrate, creatinine and dicyandiamide in the creatine powder at the same time, It is beneficial to realize the effective control of the quality of creatine powder.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to a content determination method for simultaneously detecting multiple components in creatine powder. Background technique [0002] Creatine powder is a chemical substance that can increase bodybuilders' lean body mass and muscle explosiveness and endurance. At the same time, creatine powder can bring water into the muscles, increasing the size of muscle cells, which is conducive to the absorption of amino acids by muscle cells. At present, the detection methods for creatine monohydrate in creatine powder include GB 24154-2015 National Food Safety Standard General Rules for Sports Nutrition Foods, and QB / T2834-2006 Edible Creatine for Sports Nutrition Foods. GB 24154-2015 and QB / T 2834-2006 only require the detection of the content of creatine monohydrate in creatine powder. [0003] However, since creatine powder will produce dicyandiamide and creatinine as by-products in the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/76
CPCG01N30/02G01N30/06G01N30/76G01N2030/065
Inventor 叶少文高芝炀苏昭仑李珍黄进丽黎小兰曾雪君
Owner BY HEALTH CO LTD
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