Canine distemper virus drying type real-time fluorescence PCR detection reagent

Abstract

The invention discloses a canine distemper virus drying type real-time fluorescence PCR detection reagent, and belongs to the technical field of detection reagents. A method comprises the following steps: a, product specification and kit components; b, reagent preparation (carried out in a reagent preparation area); c, sample treatment and sample addition (carried out in a sample treatment area);d, PCR detection (carried out in a nucleic acid amplification region); and e, result analysis and determination. A sample is detected to be positive or negative by collecting fluorescence signals, theefficiency is high, operation is easy, the detection result needs to meet the necessary conditions, the characteristics of low cost, convenience, rapidness and low test condition requirement are achieved, the detection efficiency is improved, and the detection accuracy is also ensured.

Description

technical field [0001] The invention relates to the technical field of detection reagents, in particular to a dry-type real-time fluorescent PCR detection reagent for canine distemper virus. Background technique [0002] Canine distemper (CD) is an acute, highly contagious infectious disease caused by canine distemper virus (CDV) infection of dogs. Canine distemper virus is highly contagious and can cause illness and even death in dogs and some other carnivorous animals. Puppies of 3 to 6 months are more susceptible to canine distemper than adult dogs. Canine distemper virus often leads to the death of dogs, so it is very necessary to detect in the early stage of similar symptoms, but the existing traditional detection methods for canine distemper virus have low test accuracy, inconvenient operation, and low efficiency. Therefore, we propose a dry-type real-time fluorescent PCR detection reagent for canine distemper virus. Contents of the invention [0003] The object o...

AI Technical Summary

Problems solved by technology

Canine distemper virus often leads to the death of dogs, so it is very necessary to detect in the early stage of similar symptoms, but the existing traditional detection methods for canine distemper virus have low test accuracy, inconvenient operation, and low efficiency. To this end, we propose a dry-type real-time fluorescent PCR detection reagent for canine distemper virus