Ion dissociation method based on quadrupole rod-ion trap tandem mass spectrometer

An ion trap and quadrupole technology, applied in the field of mass spectrometry, can solve problems such as application limitations, achieve the effects of reduced storage capacity, time saving, and improved analysis speed

Active Publication Date: 2019-12-24
NAT INST OF METROLOGY CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, no matter what kind of ion trap, there is a space charge effect, which leads to the effective capture and mass analysis of the target mass-to-charge ratio ion (hereinafter referred to as the target ion) by the impurity ion in the actual analysis application, and the application is greatly limited.

Method used

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  • Ion dissociation method based on quadrupole rod-ion trap tandem mass spectrometer
  • Ion dissociation method based on quadrupole rod-ion trap tandem mass spectrometer
  • Ion dissociation method based on quadrupole rod-ion trap tandem mass spectrometer

Examples

Experimental program
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Effect test

Embodiment 1

[0042]1ppm reserpine was injected into the mass spectrometer at a flow rate of 5uL / min, and was ionized using an electrospray ionization source (ESI), and the ion capture time was about 10ms. The value of q was chosen as 0.3. A certain radio-frequency (radio-frequency, RF) alternating electric field and direct current (DC) electric field are applied to the quadrupole, so that the target ions can be screened out. The target ions then enter the ion trap where they are stored. Finally, the target ions are ejected by applying an excitation electric field, and the detection of the target ions is completed by the detection equipment. The detection of the target ions is as follows: Figure 5 shown.

[0043] 1ppm reserpine was injected into the mass spectrometer at a flow rate of 5uL / min, and was ionized using an electrospray ionization source (ESI), and the ion capture time was about 10ms. The value of q was chosen as 0.3. A certain radio-frequency (radio-frequency, RF) alternati...

Embodiment 2

[0048] Two peptides with the same mass number but different structures were prepared in the laboratory, namely VVFFGG and GVFGVF, both of which have a mass-to-charge ratio of 625, and the mass-to-charge ratio of the characteristic fragment ion of the target ion VVFFGG is 460.4. 10 ml of the 20 μmol / L solutions were taken and mixed to obtain 20 ml of mixed solutions with respective concentrations of 10 μmol / L.

[0049] It was ionized using an electrospray ionization source (ESI), the ion capture time was about 500ms, the q value was selected as 0.3, and the injection speed was 5uL / min. A certain radio-frequency (radio-frequency, RF) alternating electric field and direct current (DC) electric field are applied to the quadrupole, so that the target ions can be screened out. The target ions then enter the ion trap where they are stored. Finally, the target ions are ejected by applying an excitation electric field, and the detection of the target ions is completed by the detection...

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Abstract

The invention relates to the technical field of mass spectrometry and provides an ion dissociation method based on a quadrupole rod-ion trap tandem mass spectrometer. A sample is ionized by an electrospray ion source, ions with a set mass-to-charge ratio are screened out from ionized ions by utilizing a quadrupole rod to enter an ion trap, the ions are dissociated by the ion trap, and dissociatedfragment ions are stored in the ion trap, wherein two processes of screening the target ions by the quadrupole rod and dissociating the ions by the ion trap are carried out at the same time. Differentfrom a traditional dissociation operation which occurs after an ion gate is closed, according to the method, a dissociation operation is applied to the ion trap while the target ions are screened bythe quadrupole rod, so that the target ions and impurity ions with similar mass-to-charge ratios are dissociated immediately when entering the ion trap, the dissociation of the target ions and the capture of the fragment ions are not interfered by the impurity ions, and the analysis accuracy is remarkably improved; meanwhile, the dissociation operation does not need to be carried out in an additional time sequence, so that the operation time is saved.

Description

technical field [0001] The invention relates to the technical field of mass spectrometry, in particular to an ion dissociation method based on a quadrupole-ion trap tandem mass spectrometer. Background technique [0002] As one of the important representatives of modern analytical instruments, mass spectrometer is a high-sensitivity and high-resolution instrument for determining the structure of substances. Its basic working principle is: first introduce the detected substance in the form of ions, separate the ions in space, time or frequency spectrum according to the mass-to-charge ratio (m / z) by electric field or magnetic field, and then pass through the ion detector These separated ions are detected, resulting in a mass spectrum. Through the analysis of the mass spectrum, the chemical composition, structure and content of the detected substance can be obtained. [0003] The quadrupole mass analyzer is structured by placing four metal cylinders in parallel on two mutuall...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): H01J49/42
CPCH01J49/426H01J49/4265H01J49/427
Inventor 江游安育廷李畅方向黄泽建戴新华谢洁龚晓云翟睿张谛
Owner NAT INST OF METROLOGY CHINA
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