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Method for improving detoxification efficiency of fructus momordicae seedlings

A technique for Luo Han Guo and seedlings is applied in the field of improving the detoxification efficiency of Luo Han Guo seeds and seedlings, which can solve the problems of unguaranteed detoxification efficiency, cumbersome detoxification culture method, unsuitable for industrialized production and the like, and achieves low production cost and culture method. Simple and efficient effect

Active Publication Date: 2020-01-07
GUILIN NATURAL INGREDIENTS CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, there are certain defects in the above-mentioned detoxification method. No matter the conventional shoot tip culture method or the detoxification and detoxification medium have to be used, only one-time detoxification is carried out, and the detoxification efficiency cannot be guaranteed; Toxic culture method is loaded down with trivial details, and production cost is high, is not suitable for industrialized production

Method used

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  • Method for improving detoxification efficiency of fructus momordicae seedlings
  • Method for improving detoxification efficiency of fructus momordicae seedlings

Examples

Experimental program
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Effect test

Embodiment 1

[0022] 1. Preparation of virus inhibitor culture medium:

[0023] Add plant growth regulator 6-BA and NAA in MS medium, make its final concentration reach 0.3ppm and 0.03ppm respectively. Add ribavirin 10 ppm after autoclaving.

[0024] 2. Detoxification pretreatment of Luo Han Guo susceptible seedlings:

[0025] Take the 1cm terminal buds of the virus seedlings and transfer them to the virus inhibitor medium for subculture once, for 10 days each time. The culture conditions are: temperature 25°C, light intensity 33umol / s / m 2 , The light time is 12 hours a day. Then transfer it to 35°C and take it out after 15 days for stripping off the shoot tip.

[0026] 3. Preparation and cultivation of shoot tips:

[0027] The virus seedlings treated with high temperature were taken out, and the shoot tip meristem of 0.3mm was stripped under a stereomicroscope, and immediately after the shoot tip was stripped, it was placed in a medium without virus inhibitors for cultivation. The cul...

Embodiment 2

[0031] 1. Preparation of virus inhibitor culture medium:

[0032] Add plant growth regulator 6-BA and NAA in MS medium, make its final concentration reach 0.7ppm and 0.07ppm respectively. After autoclaving, 100 ppm of morpholinidine was added.

[0033] 2. Detoxification pretreatment of Luo Han Guo susceptible seedlings:

[0034] Take the 1cm terminal buds of the virus seedlings and transfer them to the virus inhibitor medium for subculture 5 times, each time for 30 days. The culture conditions are: temperature 30°C, light intensity 33umol / s / m 2 , The light time is 12 hours a day. Then transfer it to 39°C and take it out after 5 days for stripping off the shoot tip.

[0035] 3. Preparation and cultivation of shoot tips:

[0036] The virus seedlings treated with high temperature were taken out, and the shoot tip meristem of 0.3mm was stripped under a stereomicroscope, and immediately after the shoot tip was stripped, it was placed in a medium without virus inhibitors for cul...

Embodiment 3

[0040] 1. Preparation of virus inhibitor culture medium:

[0041] Add plant growth regulator 6-BA and NAA in MS medium, make its final concentration reach 0.5ppm and 0.05ppm respectively. Add Tamiflu 20ppm after autoclaving.

[0042] 2. Detoxification pretreatment of Luo Han Guo susceptible seedlings:

[0043] Take the 1cm terminal buds of the virus seedlings and transfer them to the virus inhibitor medium for subculture for 3 times, each time for 20 days. The culture conditions are: temperature 30°C, light intensity 33umol / s / m 2 , The light time is 12 hours a day. Then transfer it to 38.5°C and take it out after 15 days for stripping off the shoot tip.

[0044] 3. Preparation and cultivation of shoot tips:

[0045] The virus seedlings treated with high temperature were taken out, and the shoot tip meristem of 0.2 mm was stripped under a stereomicroscope, and immediately after the shoot tip was stripped, it was placed in a medium without virus inhibitors for cultivation. ...

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Abstract

A method for improving the detoxification efficiency of fructus momordicae seedlings includes: step 1, preparing a rapid fructus momordicae propagation culture medium containing a virus inhibitor; step 2, transferring the fructus momordicae seedlings into the culture medium of the step 1 to subculture at 25-30 DEG C; step 3, cultivating the fructus momordicae seedlings of the step 2 at 35-39 DEG C, and cutting off stem tips of the seedlings; and step 4, inoculating a common rapid propagation culture medium with the stem tips of the fructus momordicae seedlings of the step 3 to obtain virus-free fructus momordicae plants. The culture medium containing the virus inhibitor at least comprises one of 10-60 ppm of virazole, 100-500 ppm of moroxydine and 20-100 ppm of oseltamivir. The method hasthe advantages that the virus inhibitor and a stem-tip detoxification method are combined to perform secondary detoxification on the fructus momordicae seedlings, and accordingly, the detoxification efficiency of the fructus momordicae seedlings is remarkably improved; the detoxification culture method is simple, the operation process is simplified, the production cost is low, and the method is suitable for industrial production.

Description

technical field [0001] The invention relates to the field of grosvenoria plant propagation, in particular to a method for improving the detoxification efficiency of grosvenoria seedlings. Background technique [0002] At present, the detoxification methods of Luo Han Guo mainly contain two: 1) conventional shoot tip culture method: take the tissue cultured seedlings of Luo Han Guo as test material, strip off the shoot tip after high-temperature heat treatment for different days, and carry out the detoxification of Luo Han Guo mosaic virus. 2) Use detoxification medium for detoxification, such as: [0003] Chinese patent CN106718922A discloses a tissue culture method for effective detoxification of Luo Han Guo, including: sterilizing the explants to obtain sterile explants; inoculating the obtained sterile explants into MS induction medium for induction culture to obtain sterile Bacteria test-tube plantlets; pre-cultivate the obtained sterile test-tube plantlets in MS pre-...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 张玉华莫雪燕兰玉
Owner GUILIN NATURAL INGREDIENTS CORP
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