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Artificial cultivation method for xylaria nigripes

A technology of artificial cultivation and Wuling ginseng, which is applied in the fields of cultivation, plant cultivation, mushroom cultivation, etc., can solve the problems of expensive and wild resource destruction prices, and achieve the effects of low cost, easy to master, and simple domestication and cultivation methods

Active Publication Date: 2020-01-10
KUNMING INST OF EDIBLE FUNGI CHINA NAT SUPPLY & MARKETING GENERAL COOP
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AI Technical Summary

Benefits of technology

This technology allows for high quality willing shens (WL), which are difficult or expensive to produce by natural methods like plantings. It also makes it easier to obtain these fruits from wild plants that have been developed over time due to their lower costs compared with traditional ways such as breeding techniques. Additionally, this process involves simpler operations than other culturing processes used on Earth, making them more efficient at producing large amounts of productive crop material quickly.

Problems solved by technology

The technical problem addressed in this patents relates to growing mushrooms like those belonging to Phyllidae family, particularly Lutrarium spinosolithoides Benthicaceae, while preserving their nutraceuticals through processing methods involving dryings, grinding, heat treatment, cooling, crushing, milling, mixing with water before use.

Method used

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preparation example Construction

[0027] The preparation method of described strain isolation medium comprises the following steps:

[0028] 1) Cut the peeled potatoes and fresh Wuling ginseng into small pieces and mix well to obtain material a, add water to material a, boil and filter to obtain filtrate b;

[0029] 2) Add glucose, agar powder, peptone, potassium dihydrogen sulfate, magnesium sulfate and vitamin B to the filtrate b, add water to the volume, stir to dissolve, subpackage, sterilize and pour the plate to obtain the target strain isolation medium .

[0030] Its specific operation is as follows:

[0031] Step 1: Cut 200 g of peeled potatoes into small pieces, 20 g of fresh Wuling ginseng, cut into small pieces, add 500ml of water and boil until rotten;

[0032] Step 2: Filter, take the filtrate, add 20g of glucose, 20g of agar powder, 5g of peptone, 2g of potassium dihydrogen phosphate, 2g of magnesium sulfate, and 1-3 tablets of vitamin B;

[0033] Step 3: Add water to make up to 1000 mL;

[0...

Embodiment 1

[0068] The formula is: 60% sawdust, 24% wheat bran, 14% wheat, 1% gypsum, 0.5% potassium dihydrogen phosphate, and 0.5% magnesium sulfate as cultivation materials. The domestication and cultivation of Wuling ginseng includes the following steps:

[0069] (1) Mix the ingredients according to the formula, and the water content is 60%;

[0070] (2) Use 650mL tissue culture bottle as the cultivation container;

[0071] (3) Compact the filling of each bottle to one-third;

[0072] (4) Sterilization, using high-pressure steam at a temperature of 121°C for 2h to 3h;

[0073] (5) Take out and cool after the sterilization is completed;

[0074] (6) Cooling and inoculation, when the temperature of the cultivation medium is cooled to about 25°C, liquid inoculation is carried out, and the liquid strain of Wuling ginseng prepared in advance is used, and the inoculation amount is 5% to 8% of the mass of the culture medium;

[0075] (7) Mycelia culture, the temperature is controlled at 24...

Embodiment 2

[0080] The formula is: 40% cottonseed husk, 20% sawdust, 14% wheat, 1% gypsum, 0.5% potassium dihydrogen phosphate, and 0.5% magnesium sulfate as cultivation materials. The domestication and cultivation of Wuling ginseng includes the following steps:

[0081] (1) Mix the ingredients according to the formula, and the water content is 60%;

[0082] (2) Use 650mL tissue culture bottle as the cultivation container;

[0083] (3) Compact the filling of each bottle to one-third;

[0084] (4) Sterilization, using high-pressure steam at a temperature of 121°C for 2h to 3h;

[0085] (5) Take out and cool after the sterilization is completed;

[0086] (6) Cooling and inoculation, when the temperature of the cultivation medium is cooled to about 25°C, liquid inoculation is carried out, and the liquid strain of Wuling ginseng prepared in advance is used, and the inoculation amount is 5% to 8% of the mass of the culture medium;

[0087] (7) Mycelia culture, the temperature is controlled ...

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Abstract

The invention discloses an artificial cultivation method for xylaria nigripes. The artificial cultivation method for the xylaria nigripes includes the steps of preparing xylaria nigripes strains, preparing liquid xylaria nigripes strains and cultivating the xylaria nigripes, and specifically includes: selecting and sterilizing fresh xylaria nigripes, cutting off internal tissues, inoculating a solid strain separation culture medium with the fresh xylaria nigripes to cultivate for tissue germination, performing purification for 1-3 times, and obtaining the target xylaria nigripes strains aftergene detection; inoculating a liquid culture medium with the xylaria nigripes strains, and performing shake cultivation to obtain the target liquid xylaria nigripes strains; and subjecting the liquidxylaria nigripes strains to liquid inoculation, performing cultivation until the xylaria nigripes is matured, and picking the xylaria nigripes. The artificial cultivation method successfully domesticates and cultivates the xylaria nigripes, and has the advantages of simplicity, sufficiency in raw materials and the like, thereby promoting further research, use and value development of the xylaria nigripes. Multiple groups of experiments prove that the cultivation method can successfully domesticate the xylaria nigripes.

Description

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Claims

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Application Information

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Owner KUNMING INST OF EDIBLE FUNGI CHINA NAT SUPPLY & MARKETING GENERAL COOP
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