A kind of cellulose degrading composite bacterial agent and its preparation method and application

A technology of cellulose degradation and compound bacterial agent, applied in the field of biological resources, can solve the problems of poor composting effect and narrow application range, and achieve good cellulose degradation effect and wide application range

Inactive Publication Date: 2021-07-09
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a cellulose-degrading composite bacterial agent and its preparation method and application. The strains used cooperate to solve the defects of narrow application range and poor composting effect of the existing bacterial agents

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The present embodiment prepares microbial inoculum agent with following method:

[0025] will contain Stenotrophomonas, Ochrobactrum, Achromobacter, Nimbya alternantherae, Guehomyces, Candida, Humic The mixed bacterial solution of Vanrija Humicola and Fusarium with a volume ratio of 1:0.064:0.98:0.01:0.39:0.039:0.029:0.01 is inoculated in LHE medium and fermented to obtain a cellulose-degrading composite bacterial agent.

[0026] LHE medium includes: peptone 9.0g / L ~ 9.5g / L, NaCl 7.0g / L ~ 7.5g / L, yeast powder 3.5g / L ~ 4.0g / L, filter paper 0.1g / L ~ 0.2g / L , NaNO 2 2g / L~2.5g / L and CaCl 2 0.1g / L~0.15g / L.

[0027] The prepared bacterial agent is in the form of bacterial liquid, including:

[0028] The viable count of Stenotrophomonas is 3.0-3.5 lg cfu / mL, the viable count of Achromobacter is 2.5-3.5 lg cfu / mL, and the viable count of Ochrobactrum is 1.0~1.2lg cfu / mL, the viable count of Alternaria (Nibya alternantherae) is 0.04lg~0.055lgcfu / mL, the viable count of cold...

Embodiment 2

[0032] In this embodiment, the manure of pigs and chickens is used to carry out fertilizer decomposing experiment to measure the change of N element composition and content before and after the fertilizer is decomposed. The specific steps are as follows:

[0033] (1) Get 10kg of pig manure and mix it with 6kg of grape twig sawdust, then add 20kg of water for composting, and measure the composition and content of N therein by the Kjeldahl method.

[0034] (2) Add the composite microbial bacterial agent 1 of 800mL embodiment 1 gained to the compost of step (1):

[0035] (3) After composting and decomposing for 30 days, the composition and content of N in the compost were determined by the Kjeldahl method.

[0036] The results showed that NH in compost before compost maturity 4 The content is 6236.2mg / L, NO 3 The content is 10.1mg / L; after the compost is decomposed, NH 4 The content is 8151.4mg / L, NO 3 The content is 15.1mg / L, of which NH 4 Increased by 30.7%, NO 3 An incre...

Embodiment 3

[0038] The steps of this embodiment are the same as those in Example 1, except that the pig manure is replaced with chicken manure.

[0039] The results showed that NH in compost before compost maturity 4 The content is 2296.3mg / L, NO 3 The content is 2.2mg / L, after the compost is decomposed, NH 4 The content is 6041.3mg / L, NO 3 The content is 7.6mg / L, of which NH 4 163% improvement, NO 3 An improvement of 245.4%.

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Abstract

The invention provides a cellulose-degrading composite bacterial agent and its preparation method and application. The cellulose-degrading composite bacterial agent includes Stenotrophomonas, Pallidum, Achromobacter, Alternaria, cold-resistant yeast, and Candida , Humicola insolens and Fusarium, the total number of viable bacteria in the inoculum should not be less than 7.2lg cfu / mL. The cellulose-degrading composite bacterial agent provided by the present invention can degrade the cellulose in the biocompost to a great extent, reduce the C / N from 25:1 to 10.03:1, and promote the transformation of N elements in the compost towards the direction that plants can use , and is suitable for biocomposting of various plant residue components, and can play a good role in cellulose degradation.

Description

technical field [0001] The invention relates to the field of biological resources, in particular to a cellulose-degrading composite bacterial agent and its preparation method and application. Background technique [0002] With the steady development of the existing grape fruit industry, the scale of production is constantly expanding and standardized, and the cultivated area is increasing year by year, and the annual waste of grape pruning branches has become an inevitable problem. With the normal fruit tree maintenance, pruning, and replacement of fruit tree seedlings every year in the grape agricultural orchard, a large number of fruit tree branches will be produced. Because the cellulose in fruit tree branches is highly complex, it degrades very slowly in the natural environment. The traditional method is to sort the discarded branches of agricultural orchards into piles, and burn them in the open air uniformly or pile them up in the garden for a long time. The accumula...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/14C12N1/16C05F15/00C05G1/00C05F17/20C12R1/01C12R1/025C12R1/645C12R1/72C12R1/77
CPCC05C9/00C05F3/00C12N1/14C12N1/16C12N1/20Y02W30/40
Inventor 胡建芳刘帆刘振华朱海峰
Owner CHINA AGRI UNIV
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