Isolation and identification method of highly purebred ultramicroalgae

Abstract

The present invention discloses an isolation and identification method of highly purebred ultramicroalgae. The method integratedly and cooperatively uses a flow cytometer and a molecular biology technology, firstly uses the flow cytometer to sort out a specific ultramicroalgae group, then conducts single-cell well plate culture, conducts expanded culture and uses a high-throughput sequencing technology to identify algae. The method can efficiently and quickly isolate and culture the highly purebred ultramicroalgae, provides a complete set of techniques for the isolation and culture of the ultramicroalgae in lake waters, and provides a part of theoretical technical supports for expansion of algae species banks in China.

Description

technical field [0001] The invention belongs to the technical field of algae separation and cultivation in the fields of environmental science and ecology, and in particular relates to a method for rapidly separating pure ultramicro algae. Background technique [0002] Ultramicroalgae have a large population and a wide range of habitats, and are generally distributed in major ocean and lake ecosystems. The advantages of their small size and large specific surface area enable them to efficiently obtain and use resources for growth and reproduction, so they play an important role in the primary productivity of lakes. The annual average contribution rate decreases with the increase of the nutrient level of the lake. According to relevant research, the contribution rate reaches 65%, 60% and 23% respectively in Fuxian Lake, Poyang Lake and Chaohu Lake. As an initial key link in the microfood cycle, ultramicroalgae can be ingested by some heterotrophic flagellates and ciliates, an...

AI Technical Summary

Problems solved by technology

Also because of the small size of the individual nanoalgae and the lack of significant morphological classification standards, it is difficult to identify the species with an optical microscope or even an electron microscope, so that few new species of the Nanoalgae have been reported, which greatly reduces people's understanding of the ultramicroalgae. cognition, thus ignoring this biological resource with various components but not to be underestimated

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