A method for detecting the content of α-konojirimycin in white tree medicinal material

Abstract

The invention provides a method for detecting the content of α-konojirimycin in white tree medicinal material. The method comprises: preparation of reference substance solution: accurately weigh α-konojirimycin reference substance, add solvent to dissolve and be mixed with the reference substance solution of required concentration; prepare test solution: take white tree medicinal material powder to be tested, add Extract the solvent to extract, get the supernatant after centrifugation, constant volume, obtain the need testing solution; Assay: by liquid chromatography-tandem mass spectrometry, measure the content of α-konojirimycin in the reference substance solution and the need testing solution The peak area was calculated according to the external standard method to calculate the content of α-konojirimycin in the white tree medicinal material. The present invention adopts liquid chromatography-tandem mass spectrometry, realizes the resolution of polyhydroxyalkaloid α-konojirimycin and its four chiral isomers for the first time, and realizes the direct characterization of α-konojirimycin , Quantitative analysis, with good system applicability, stability, repeatability and reproducibility.

Description

A method for detecting the content of α-konojirimycin in Baishu medicinal materials technical field The present invention relates to the detection field of Chinese medicinal material content, be specifically related to the content of α-konojirimycin in a kind of white tree medicinal material Detection method. Background technique White tree, the Latin name is Suregade glomerulata (BL.), belongs to Euphorbiaceae White Tree genus, tree, height 2-13 meters, Branchlets grayish black or brown. Existing studies have proved that the extract of Baishu can specifically inhibit α-glucosidase and has a very high Strong activity, beneficial to the improvement of glucose metabolism and lipid metabolism disorders in diabetes, and can be used for the treatment of diabetes and impaired glucose tolerance treatment. The azasaccharide-type polyhydroxyalkaloids represented by the α-konojirimycin structural parent nucleus are extracted from the white tree. The isolated natural α-gluc...

AI Technical Summary

Problems solved by technology

[0004] Therefore, the technical problem to be solved by the present invention is to overcome the defects of inaccurate detection results caused by the cumbersome reaction procedures, high risk and insufficient resolution ability of the content detection method of α-konojirimycin in the prior art, thereby providing a Determination method of α-takanojirimycin in the medicinal material of Baishu with simple operation, rapidity, environmental friendliness and ability of isomer resolution

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