Method for researching toxicity effect of jujube nectar on bees based on intestinal flora

Abstract

The invention belongs to the technical field of biotechnology and agriculture, and provides a method for researching the toxic effect of jujube flower nectar on bees based on intestinal flora for thecurrent situation that the toxic effect of jujube nectar on bees cannot be explained in the existing research. Based on changes of microbial intestinal flora of bees and functional analysis of differential bacteria by the jujube nectar, the occurrence mechanism of the jujube nectar to the jujube blossom diseases of bees is excavated, and a basis is provided for prevention and treatment of the jujube blossom diseases of bees. Microbial DNA in intestinal feces of bees is collected, the regulation of jujube nectar on intestinal flora of bees is researched by using a Hiseq2500 PE250 sequencing platform and based on a bacterium 16s rDNA V3-V4 variable region, and the up-regulated or down-regulated bacterium genus in a jujube flower disease group is determined. The mechanism that the jujube nectar inhibits biodegradation and detoxification pathways of exogenous substances by changing intestinal flora, up-regulates biosynthesis of alkaloid amino acids and generates toxic effects on bees is clarified, and the method is applied to target selection in prevention and treatment of jujube blossom diseases through bees.

Description

technical field [0001] The invention belongs to the fields of biotechnology and agricultural technology, and in particular relates to a research method for jujube flower nectar-induced toxicity to bees based on intestinal flora. Background technique [0002] Bee jujube flower disease, also known as bee jujube poisoning, occurs in the nectar-flowing period of jujube trees, and is an endemic disease in the nectar-flowing period of jujube flowers in North China. After honey bees collect jujube nectar, their abdomens swell, they lose their ability to fly, their wings stretch out or stand up, and they jump and crawl outside the nest door. die. After the onset, a large number of collecting bees die, and the bee colony declines. In the year of major occurrence, the bee colony can be reduced by 1 / 3-1 / 2, which seriously affects the production of honey in the jujube flowering period. [0003] There are few studies on the pathogenesis of bee jujube disease. It is generally believed t...

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a kind of jujube nectar based on intestinal flora to cause The research method of bee toxicity effect; based on the change of jujube flower nectar on the microbial intestinal flora of bees and the function analysis of different bacteria, to dig out the mechanism of jujube flower nectar on bee jujube disease, and provide a basis for the prevention and treatment of bee jujube disease

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