Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Construction method of RANKL recombinant protein expression vector and application thereof

A technology for expressing vectors and recombinant proteins, which can be used in vectors, applications, nucleic acid vectors, etc., and can solve problems such as abnormal osteoclast function.

Pending Publication Date: 2020-06-16
NANTONG UNIVERSITY
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, the treatment of osteosclerosis and periapical edema is mainly limited to traditional medicine or microsurgery, but patients with severe osteosclerosis caused by autosomal abnormalities cannot achieve good results, mainly due to the In the metabolic process, RANKL is caused by the abnormal function of osteoclasts; in the current study, it is shown that RANKL plays an important role in regulating the differentiation and activation of osteoclasts; and the occurrence of bone metabolic diseases is mostly related to the bone formation and osteoclasts mediated by osteoblasts. Cell-mediated bone resorption is closely related, however, how to construct RANKL is not yet clear

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Construction method of RANKL recombinant protein expression vector and application thereof
  • Construction method of RANKL recombinant protein expression vector and application thereof
  • Construction method of RANKL recombinant protein expression vector and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0063] In order to make the technical problems, technical solutions and advantages to be solved by the present invention clearer, the following will describe in detail with reference to the drawings and specific embodiments.

[0064] A method for constructing a RANKL recombinant protein expression vector, comprising the following processes: (1) in vitro molecular cloning technology to obtain the full length of the mouse RANKL gene; (2) through sequencing, it is confirmed that the obtained cloned sequence is completely consistent with the sequence in the database; Further, the mouse RANKL gene was recombined into the pET-32a expression vector by gene recombination technology, and the RANKL recombinant protein expression vector was successfully constructed.

[0065] Specifically, a method for constructing a RANKL recombinant protein expression vector comprises the following steps:

[0066] (1) In vitro molecular cloning technology to obtain the full length of mouse RANKL gene; ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a construction method for an RANKL recombinant protein expression vector, which is characterized by comprising the following steps of: (1) obtaining a full length of a mouse RANKL gene by using an in-vitro molecular cloning technology; (2) determining that the obtained clone sequence is completely consistent with the sequence in the database through sequencing; furthermore,recombining the mouse RANKL gene into a pET-32a expression vector through a gene recombination technology, and successfully constructing the RANKL recombinant protein expression vector. The inventionalso provides an application of the RANKL recombinant protein expression vector in preparation of drugs for treating diseases related to abnormal bone metabolism; wherein the bone metabolism abnormality related diseases comprise osteosclerosis and periapical caries and odontocele. According to the RANKL recombinant protein expression vector disclosed by the invention, a large amount of RANKL recombinant proteins can be induced and expressed in vitro, and finally, the differentiation and bone absorption functions of osteoclasts are promoted through the RANKL recombinant proteins, so that osteosclerosis and periodontal cardiopathy are treated and improved.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a construction method and application of a RANKL recombinant protein expression vector. Background technique [0002] At present, the treatment of osteosclerosis and periapical edema is mainly limited to traditional medicine or microsurgery, but patients with severe osteosclerosis caused by autosomal abnormalities cannot achieve good results, mainly due to the In the metabolic process, RANKL is caused by the abnormal function of osteoclasts; in the current study, it is shown that RANKL plays an important role in regulating the differentiation and activation of osteoclasts; and the occurrence of bone metabolic diseases is mostly related to the bone formation and osteoclasts mediated by osteoblasts. Cell-mediated bone resorption is closely related, however, how to construct RANKL is not yet clear. Contents of the invention [0003] The purpose of the present inve...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/85C12N15/12C12N15/10A61K38/17A61K48/00A61P1/02A61P19/08
CPCC12N15/85C07K14/70575C12N15/1096A61K38/177A61K48/005A61P19/08A61P1/02C12N2800/107C12Q2531/113C12Q2521/107
Inventor 王东闵雯嫣
Owner NANTONG UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com