Multichannel exposure contamination method for in-vitro smoke smoking

A multi-channel, flue gas technology, applied in the field of flue gas exposure and poisoning experiments, can solve problems such as inaccurate evaluation of flue gas exposure doses, and achieve effective evaluation results

Active Publication Date: 2020-06-30
ZHENGZHOU TOBACCO RES INST OF CNTC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The object of the present invention is to provide a multi-channel exposure method for in vitro smoking smoke exposure to solve the problem of inaccurate evaluation of the smoke exposure dose of the existing Vitrocell smoke exposure system

Method used

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  • Multichannel exposure contamination method for in-vitro smoke smoking
  • Multichannel exposure contamination method for in-vitro smoke smoking
  • Multichannel exposure contamination method for in-vitro smoke smoking

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] In the multi-channel exposure method of in vitro smoking smoke exposure in this embodiment, the above-mentioned smoke exposure device is used to perform multi-channel exposure exposure of Beas-2b cells in vitro smoking smoke, and the smoke smoked in vitro passes through without dilution with air. The flue gas transmission and diversion device is evenly divided into 3 exposure chambers for three-channel smoke exposure exposure, and the exposure dose is calculated by formula (1). Among them, Z is 3 puffs, C is the maximum number of cigarette puffs 8, and I=1 / 3.

[0057] Close the flue gas discharge control valve 13, fully open the gas discharge control valve 14 and exhaust gas discharge control valve 17 connected to the three exposure chambers, and set the flow rate of the air pump to 20 mL / min. At this time, the number of layers of the distribution pipeline of the flue gas transmission and distribution device is 1, and the number of distributions (the number of actual wo...

Embodiment 2

[0070] On the basis of the above-mentioned flue gas exposure device, the flue gas transmission and diversion device is designed as a two-level diversion pipeline mode to simulate the diversion of flue gas in the tracheal tree.

[0071] In the multi-channel exposure method of extracorporeal smoking smoke in this embodiment, the above-mentioned two-level shunt pipeline is used to simulate the shunting of smoke in the two-level tracheal tree. The road is divided into 2 roads (the smoke is divided into 2 roads through the left and right bronchi when simulating human smoking), 1 road is connected to the smoke collector (collecting the smoke), and 1 road is connected to the structure of 1 split and 4 shunts. The 3-way flue gas separated by the 1-splitting 4-splitting structure is respectively connected to 3 exposure chambers, and the remaining 1-way flue gas is connected to the flue gas trap.

[0072]According to the above shunt transmission of smoke, smoke 0, 2, 4, 6, and 8 mouthfu...

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Abstract

The invention belongs to the field of smoke exposure experiments, and particularly relates to a multichannel exposure contamination method for in-vitro smoke smoking. The method comprises the following steps: smoke smoked in vitro is shunted into a plurality of exposure bins through a smoke transmission shunting device without being diluted by air and undergoes multichannel smoke exposure contamination, and the contamination dosage is calculated according to the number of cigarettes and the number of smoking ports or the number of cigarettes and the number of smoking ports corresponding to thecontent of total particulate matters and nicotine in mainstream smoke. According to the in-vitro smoke smoking multichannel exposure contamination method, smoke generated by cigarette smoking is notdiluted and is averagely shunted into the multiple exposure bins through the smoke transmission shunting device, the contamination dosage evaluation does not need to consider the influence of air dilution, and the contamination dose of the exposure bins can be evaluated more accurately and effectively.

Description

technical field [0001] The invention belongs to the field of smoke exposure and poisoning experiments, and in particular relates to a multi-channel exposure and poisoning method for smoked smoke outside the body. Background technique [0002] The whole smoke exposure method has become an important exposure method for assessing smoke toxicity or biological effects in vitro. The smoke exposure device on which this exposure method is based consists of a smoking machine, a smoke delivery system, and an exposure chamber capable of exposing the gas-liquid interface. The smoke generated by the smoking machine enters the exposure chamber through the smoke delivery system, and the cells in the exposure chamber are poisoned, and the toxicity or biological effects of the smoke are evaluated according to the results of the cell exposure. [0003] At present, commercially used smoke exposure devices such as Vitrocell and Cultex mainly realize smoke exposure by diluting smoke with air gr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50
CPCG01N33/5014
Inventor 华辰凤谢复炜李翔尚平平赵俊伟秦亚琼彭斌赵阁王昇刘惠民
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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