circRNA small molecular marker indicating key pathways of healthy aging and application

A marker and small molecule technology, applied in the field of biomedicine, can solve the problems of unindustrialization and complex technical means.

Inactive Publication Date: 2020-07-03
KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

With the current technical means, the technical means to evaluate the changes of each pathway are very complicated, involving a large number of precise experimental means, and it is impossible to carry out industrialization and process operation

Method used

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  • circRNA small molecular marker indicating key pathways of healthy aging and application
  • circRNA small molecular marker indicating key pathways of healthy aging and application
  • circRNA small molecular marker indicating key pathways of healthy aging and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Key pathways of healthy aging based on circRNA small molecule markers and circRNA small molecule markers indicating the key pathways of healthy aging

[0023] Through the whole transcriptome sequencing of the total RNA of peripheral blood cells of long-lived elderly in Hainan, my country, the CIRCexplorer software was used to identify circRNAs in the data, and the co-expression analysis of circRNAs and genes was further performed. After screening (cor≥0.6&adjusted p<0.01), 40 There is a high correlation between the circRNA small molecule markers and some genes. Next, KEGG pathway enrichment analysis was performed on these genes, and 8 key pathways related to healthy aging based on circRNA small molecule markers were found.

[0024] Among them, 40 circRNA small molecule markers and their nucleotide sequences are shown in Table 1.

[0025] Table 1 The names, locations and sequences of 40 circRNAs indicating key pathways of healthy aging

[0026]

[0027]

...

Embodiment 2

[0042] Example 2 Using hsa_circ_0003296 as a single marker to evaluate the key pathways of healthy aging:

[0043] The specific implementation method is as follows:

[0044] 1. Obtain fresh anticoagulant blood samples from the subjects and perform pretreatment: After collecting the subjects' blood samples according to standardized and reasonable operating procedures, extract the total RNA of white blood cells.

[0045] 2. Remove rRNA from the obtained RNA sample, and prepare the sample according to the illumina RNA-seq library preparation manual. After the preparation is completed and passed the quality inspection, it will be sequenced on the Illumina Hiseq-2500 platform. After the low-quality filtering of the sequencing data, it was compared with the reference genome of the hg19 version, and the comparison results were identified according to the CIRCexplorer software workflow, and then the expression of the circRNA was quantified (normalized in RPM, that is, every hundred 1...

Embodiment 3

[0048] Example 3 hsa_circ_0122600 is a single marker for diagnosis of key pathways of healthy aging:

[0049] The determination of the expression level of the subject hsa_circ_0122600 is the same as Step 1-2 in Example 2. The expression level of hsa_circ_0122600 in the blood of the subjects measured above was compared with the data of the general population (115 cases of the control group). Taking a sample as an example, the hsa_circ_0122600 expression value RPM is 0.21, which is higher than the threshold of the general population (RPM=0.125), and the ribosome (Ribosome) signal of the subject is up-regulated through query table 3. From the query in Table 2, it can be seen that the change direction of the ribosome pathway is opposite to that corresponding to healthy aging, that is, the internal ribosome pathway of healthy aging will decrease as a whole. Therefore, it can be judged that the subject has poor characteristics in the ribosome functional pathway, and early warning s...

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Abstract

The invention discloses a circRNA small molecular marker indicating key pathways of healthy aging and application. The key pathways of healthy aging include 8 key pathways in 3 categories. The circRNAsmall molecular marker includes 40 circRNA and nucleotide sequences of the 40 circRNA are shown as SEQ ID NO.1-40, the circRNA small molecular marker can be directly applied to indicate the changesof the healthy aging-related pathway changes in the current state of ordinary aging people, and the health aging state is evaluated from the mechanism.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a circRNA small molecule marker indicating a key pathway of healthy aging and its application. Background technique [0002] At present, the problem of population aging in China is extremely urgent and severe. The support problems derived from the aging problem and the high medical insurance costs for senile diseases are an extremely heavy burden on individuals and the country. Therefore, research on the prevention and treatment of senile diseases and healthy aging is a very important task. Through the prediction and evaluation of functional pathways related to healthy aging, it is one of the important foundations in the scientific research of healthy aging to further indicate the intrinsic healthy aging state of the aging population. [0003] The research on healthy aging is currently mainly carried out from the perspective of a single gene, but this process is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883
CPCC12Q1/6883C12Q2600/158C12Q2600/178
Inventor 孔庆鹏葛明侠李功华
Owner KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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