Method and test kit for detecting novel coronavirus
A coronavirus and kit technology, applied in the field of biomedicine, can solve problems such as false negatives, and achieve the effect of getting rid of dependence and achieving absolute quantification.
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[0031] Example 1
[0032] The combination design of primers and probes for the new coronavirus digital PCR detection system and the establishment of the digital PCR detection system and method.
[0033] 1.1 Primer probe design:
[0034] According to the sequence of the new human coronavirus and the sequence of the β-actin gene, the following primer and probe combinations (SEQ ID NO.1-3; SEQ ID NO.4-6) for the new coronavirus digital PCR detection system were designed:
[0035] Novel coronavirus upstream primers:
[0036] 1AB-F: 5'-CAAGGTAAACCTTTGGAATTTG-3' (SEQ ID NO.1)
[0037] Novel coronavirus downstream primers:
[0038] 1AB-R: 5'-TTGTCCTCACTGCCGT-3' (SEQ ID NO. 2)
[0039] Novel coronavirus probe: 1AB-P: 5'-FAM-TGCCACTTCTGCTGCTCTTCAACC-BHQ1-3' (SEQ IDNO.3)
[0040] Upstream primer of internal reference gene β-actin:
[0041] ACTB-F: 5'-GTCTTCCCCTCCATCGTG-3' (SEQ ID NO.4)
[0042] Downstream primer of internal reference gene β-actin:
[0043] ACTB-R: 5'-GTACTTCAGGGTGAGGATGC-3' (SEQ ID NO. ...
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