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Multi-detection immune reagent and preparation method, kit, system and application thereof

An immune reagent and multiple detection technology, applied in the field of immune detection, can solve the problems of high price and limited application of multiple immune detection

Pending Publication Date: 2020-07-07
SHENZHEN DYMIND BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the high price of such encoded microsphere...

Method used

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  • Multi-detection immune reagent and preparation method, kit, system and application thereof
  • Multi-detection immune reagent and preparation method, kit, system and application thereof
  • Multi-detection immune reagent and preparation method, kit, system and application thereof

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preparation example Construction

[0056] Please refer to Figure 5 , Figure 5 It is a schematic flow diagram of an embodiment of a method for preparing an immunological reagent for multiple detection in the present invention, wherein the method includes steps:

[0057]S510, providing a preset type of first immunoconjugate, which is respectively used to detect different analytes.

[0058] In step S510, the type of the first immunoconjugate corresponds to the type of the detection index, that is, corresponds to the number of items requiring joint inspection. Wherein, the first immunoconjugates of preset types can respectively bind to corresponding antigens, and corresponding indicators have been detected.

[0059] S520. Add first fluorescent markers to each of the first immune conjugates, so that the first fluorescent markers respectively label each of the first immune conjugates.

[0060] In step S520, in order to make different kinds of first immunoconjugates have different fluorescence intensities, each o...

Embodiment 1

[0079] 1. A kit for joint detection of cTnI, CK-MB, and Myo

[0080] (1) The first immune reagent includes cTnI antibody, CK-MB antibody and Myo antibody connected to the magnetic ball, and the fluorescence intensity MF1 of FITC fluorescein connected on the cTnI antibody, CK-MB antibody and Myo antibody is respectively 25622, 48955, 89542.

[0081] (2) The second immunization reagent includes the first reagent and the second reagent. The first reagent includes biotin-labeled cTnI antibody, biotin-labeled CK-MB antibody and biotin-labeled Myo antibody, the concentration of the first reagent is 1ug / ml; the second reagent includes streptavidin-labeled phycoerythrin For protein, the concentration of the second reagent is 5ug / ml.

[0082] 2. The preparation method of the first immune reagent and the second immune reagent

[0083] (1) Preparation of the first immune reagent

[0084] Fluorescein-conjugated antibodies:

[0085] 1) Dilute cTnI antibody solution 1, CK-MB antibody s...

Embodiment 2

[0135] 1. A kit for joint detection of cTnI, CK-MB, and Myo

[0136] (1) The first immunization reagent includes cTnI antibody, CK-MB antibody and Myo antibody connected on the magnetic ball, and the surface of cTnI antibody, CK-MB antibody surface and Myo antibody surface are respectively connected with surface amino-modified and particle diameter of 80nm Silica microspheres, and the fluorescence intensity MF1 of CY5-NHS EASTER fluorescein linked to cTnI antibody, CK-MB antibody and Myo antibody were 25622, 48955, and 89542, respectively.

[0137] (2) The second immunization reagent includes the first reagent and the second reagent. The first reagent includes biotin-labeled cTnI antibody, material-labeled CK-MB antibody and material-labeled Myo antibody, the concentration of the first reagent is 1ug / ml; the second reagent includes streptavidin-labeled phycoerythrin For protein, the concentration of the second reagent is 5ug / ml.

[0138] 2. The preparation method of the firs...

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Abstract

The invention discloses a multi-detection immune reagent, a preparation method, a kit, a system and application thereof; the immune reagent comprises a plurality of first immune compositions, and eachfirst immune composition comprises: a solid phase carrier; the first immune conjugate that is used for carrying out specific immune reaction; the first fluorescent marker that is connected to the first immune conjugate, wherein each of the first immune compositions has a different fluorescence intensity. By means of the mode, dependence of the multiple detection process on the types and performance of the magnetic beads can be reduced, and the production cost is reduced.

Description

technical field [0001] The invention relates to the technical field of immune detection, in particular to a multiple detection immune reagent and its preparation method, kit, system and application. Background technique [0002] With the continuous development of immunoassay technology, multiplex immunoassay technology has become a research hotspot because it can effectively improve the detection efficiency. The so-called multiple detection technology is to add a sample to the detection device to obtain the detection results of multiple analytes at the same time. [0003] At present, for multiple immunoassay techniques, immunoconjugates for detecting different analytes are usually coupled to coded microspheres with different fluorescent types or fluorescence intensities, and by classifying the fluorescent types or intensities on the coded microspheres, it can be determined Test results for different analytes. However, the high price of such encoded microspheres greatly lim...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N33/58
CPCG01N33/54393G01N33/54313G01N33/54333G01N33/582
Inventor 蔡敏马志亚邝俊韬刘治志
Owner SHENZHEN DYMIND BIOTECH
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