Improvements in or relating to cell analysis
A cell and single-cell technology, applied in the field of cell analysis, can solve problems such as limited capacity, and achieve the effect of improving prognosis and reducing disease burden
Pending Publication Date: 2020-07-24
UNIV OF WESTERN AUSTRALIA
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Problems solved by technology
Despite its advantages, FISH-IS is limited in its ability to analyze each sample, leaving much room for improvement in terms of sensitivity and variability
Method used
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example 2
[0418] Example 2: Immuno-flowFISH compared to earlier state-of-the-art
[0419] The following example shown in Table 9 compares the Immuno-flow-FISH technique with the Immuno-S-FISH technique. Table 10 provides the broad working range for the analysis of peripheral blood mononuclear cell (PBMC) preparations, as well as highly preferred optimal conditions for this method.
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Abstract
The present invention provides a method for cell analysis, comprising: preparing a blood sample comprising nucleated cells having surface, cytoplasmic or nuclear antigens (markers); antibody stainingthe cell markers; fixing and permeabilising the cells; FISH probe hybridising to chromosomes in the cells; performing imaging flow cytometry on the cells; analysing data obtained from performing imaging flow cytometry; and diagnosing, prognosing or monitoring a medical condition based on the data analysis.
Description
technical field [0001] The present invention relates to cell analysis. In a specific non-limiting aspect, the invention relates to diagnostic and prognostic methods for analysis of tissue samples and / or cells. For example, the invention provides assays for the diagnosis, prognosis or monitoring of medical or therapeutic conditions. Background technique [0002] The following discussion of the background art is intended only to facilitate an understanding of the present invention. The discussion is not an admission that any of the material referred to is or was part of the common general knowledge before the priority date of the application. [0003] Cytogenetic analysis is an integral part of the evaluation of many diseases such as malignancies, syndromes with abnormal chromosome numbers such as trisomy 21 (Down syndrome), and mosaicism in transplant recipients. [0004] Diagnostic laboratories currently use fluorescence in situ hybridization (FISH) analysis of cells on s...
Claims
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IPC IPC(8): C12Q1/6841C12Q1/6827G01N1/30G01N21/64G01N33/53
CPCC12Q1/6841G01N33/5091G01N33/582G01N1/30C12Q1/6827G01N21/6486C12Q2565/626C12Q2563/107C12Q2565/102G01N33/5094G01N33/57426G01N2015/1006G01N15/147G01N15/1433G01N21/64G01N33/53
Inventor 温爱博凯瑟琳·阿曼达·富勒许若霖
Owner UNIV OF WESTERN AUSTRALIA