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Screening method of receptor cattle

A screening method and receptor technology, applied in the fields of medical science, material testing products, animal delivery, etc., can solve the problem of lack of progesterone detection methods, and achieve the effect of improving pregnancy rate and production efficiency

Inactive Publication Date: 2020-09-15
SHANGHAI INST OF MEDICAL GENETICS SHANGHAI CHILDRENS HOSPITAL
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the routine and common operation in the production process of animal husbandry is still to use manual observation methods such as observation of climbing behavior, emotional agitation, pudendal mucus, etc. to determine the estrus, and there is a lack of a detailed system that can effectively use progesterone detection methods Research

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  • Screening method of receptor cattle
  • Screening method of receptor cattle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Detection of serum progesterone concentration in natural estrous cycle of empty pregnant cows

[0033] Behavior of open cows was observed manually. When the cattle showed signs of climbing behavior, emotional agitation, and genital mucus to determine the estrus, the day was defined as 0d, blood was collected to detect the concentration of progesterone, and then blood was collected for detection on 5d, 10d, 15d and 20d.

[0034] Test results such as figure 1 As shown, the change rule of serum progesterone concentration in the estrus cycle of empty pregnant cows, the serum progesterone concentration of cows at 0 day of estrus was at the lowest value (≤0.64nmol / L), and then gradually increased; the progesterone concentration of 5 days of estrus was about 4.83 nmol / L; reached the peak around the 10th day of estrus, and the progesterone concentration was about 9.09nmol / L; after 15 days of estrus, the progesterone concentration began to drop rapidly, and entered th...

Embodiment 2

[0035] Example 2 Detection of Serum Progesterone Concentration in Cow Breeding or Artificial Insemination Pregnancy Period Manually observe the behavior of cows, perform mating on 0 day of estrus, and diagnose pregnancy by rectal palpation at 60 days of pregnancy, and in early pregnancy on the 20th day, the first day On the 40th day, on the 60th, 90th, and 120th day of the second trimester, and on the 180th, 240th, and 270th day of the third trimester, blood was collected from the tail vein to detect the serum progesterone concentration.

[0036] Depend on figure 2 It can be seen that the progesterone concentration of the cows that were successfully pregnant and gave birth was the lowest (≤0.64nmol / L) on the day of breeding, and then gradually increased. After 15 days, there was no decrease in the progesterone concentration, and the progesterone concentration remained at a high level throughout the pregnancy ; Although there were slight fluctuations, there was no significant ...

Embodiment 3

[0038] Example 3 Detection of Serum Progesterone Concentration in Estrus, Transplantation and Pregnancy of Embryo Transfer Recipient Cattle

[0039] Selection of recipient cattle:

[0040] The estrus behavior of cattle was manually observed, and the experimental group collected blood at 0 and 5 days of estrus to measure serum progesterone concentration, further assisting in judging the estrus time of cattle, combined with manual touch of the corpus luteum, and selecting suitable recipient cattle for simultaneous embryo transfer.

[0041] Based on the above research on progesterone changes in pregnancy of estrus cows and mating cows, it was found that the progesterone concentration of normal estrus cows was ≤0.64nmol / L on day 0 of estrus, and the concentration of progesterone on day 5 was concentrated at about 5nmol / L. Therefore, we initially screened the cows with progesterone ≤ 0.64nmol / L on day 0 and progesterone 2-8nmol / L on day 5 as suitable recipients for embryo transfer....

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Abstract

The invention discloses a screening method of receptor cattle. Cattle with progesterone less than or equal to 0.64 nmol / L in 0 day of oestrus and with progesterone of 2-9 nmol / L in 4-8 days of oestrusis selected as a proper receptor cattle for embryo transplantation. According to the method disclosed by the invention, based on systematic determination of the progesterone levels in the blood of anaturally-estrus non-pregnant cattle, a mating cattle and the embryo transplantation receptor cattle in different reproductive cycles, the oestrus condition of cattle is detected and tracked, a propertransplantation receptor cattle is selected, pregnancy conditions of the mating or the embryo transplantation receptor cattle are monitored, a standardized detection and tracking method is established, and a reference basis is provided for improving the pregnancy rate and the production efficiency of the mating or embryo transplantation receptor cattle.

Description

technical field [0001] The invention belongs to the technical field of modern animal husbandry production, and in particular relates to a screening method for recipient cattle. Background technique [0002] The estrous cycle of mammals has a very common law. It has become normal to assist the practice of regenerative medicine by detecting changes in reproductive hormones. The research and efficient application of modern animal husbandry production and embryo engineering are also inseparable from accurate testing method. The technical means of accurately judging the estrus state of cows is one of the prerequisites for the success of artificial insemination and embryo transfer of in vitro fertilization of dairy cows. In embryo transfer experiments, the synchronization of embryos and recipients is the key to the successful establishment of pregnancy. One of the basic conditions for successful embryo transfer is to maintain the consistency of the recipient cow's physiological ...

Claims

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Application Information

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IPC IPC(8): A61D17/00G01N33/74
CPCA61D17/002A61D17/006A61D17/004G01N33/74
Inventor 曾凡一曾溢滔黄淑帧邱瑾
Owner SHANGHAI INST OF MEDICAL GENETICS SHANGHAI CHILDRENS HOSPITAL
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