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A molecular marker that affects chicken shin skin color and its application

A technology of molecular marking and skin color, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of inability to identify skin color, etc., to reduce breeding time, deepen skin yellowness, Increases the effect of genetic progression

Active Publication Date: 2021-07-09
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Patent CN201010531819.2 provides a molecular marker related to chicken skin color and its identification method and application, but it is aimed at the color of the whole chicken skin, not for the identification of the shin color

Method used

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  • A molecular marker that affects chicken shin skin color and its application
  • A molecular marker that affects chicken shin skin color and its application
  • A molecular marker that affects chicken shin skin color and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Primer Design

[0036] 1. Experimental method

[0037] Find the sequence of the 3' non-coding region of the ALDH1A3 gene from https: / / www.ncbi.nlm.nih.gov / , and design a primer pair. The primer pair information is shown in Table 1 (primer sequence 5'→3'). The sequence of the primer pair was sent to Guangzhou Sangon Biotech Co., Ltd. for synthesis, and the primer pair was used to perform PCR on tissue DNA to test the specificity of the primers.

[0038] Table 1 Primer information for SNP screening of ALDH1A3

[0039]

[0040] 2. Experimental results

[0041] The results are attached figure 1 shown. Use this primer pair to carry out PCR on blood DNA, as attached figure 1 As shown, the primers are specific

Embodiment 2

[0042] Example 2 DNA pool sequencing

[0043] 1. Experimental method

[0044] The sample chickens were selected from 600 120-day-old Nanhai Jute Chicken No. 1 pure line of Jiangfeng Chicken Farm. All chickens were hatched in the same batch, raised in a flat way, and fed with corn-soybean meal that meets international formula standards feed. Use a 2ml syringe to collect blood from the subwing vein, collect the blood and store it in a centrifuge tube containing 20% ​​EDTA for blood DNA extraction.

[0045] Genomic DNA of all individuals based on Plant Mini Kit (Qiagen, Hilden, CA; Cat#69104) was used in the kit instructions to extract blood DNA, measure the quality and concentration, dilute to 50 ng / μL, and store at 4°C for later use.

[0046]30 DNA samples were randomly selected from the total extracted DNA samples to construct a mixed pool, and every three samples (about 0.33 μL of each sample) were mixed into a mixed sample, a total of 10 samples. The mixed pool samples ...

Embodiment 3

[0049] Example 3 Determination of genotype determination of ALDH1A3 mutation site g.703T>C

[0050] 1. Experimental method

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Abstract

The invention discloses a molecular marker that affects the skin color of chicken shanks and an application thereof. Chicken ALDH1A3 gene 3' non-coding region sequence g.170A>G molecular marker was used as a detection site to identify chicken shin skin color, and the yellowness of shin skin of individuals with AA genotype was greater than that of individuals with AG and GG genotypes. Using the molecular marker for marker-assisted selection can greatly enhance the yellowness of the shin skin of chickens after slaughter, and improve the uniformity of the yellowness of the shin skin of chickens. Through the molecular marker and primer pair, an efficient and accurate molecular marker-assisted breeding technology can be established, and it can be applied to the genetic improvement of the yellowness of the skin of the shank of the chicken after slaughter, so as to deepen the yellowness of the skin of the shank of the chicken after slaughter and improve the quality of the chicken. The uniformity of the yellowness of the skin of the shank is conducive to promoting the sales of chickens, especially the sales of chicken feet, increasing the sales of the enterprise and increasing the core competitiveness.

Description

technical field [0001] The invention relates to the technical field of molecular breeding, in particular to a molecular marker affecting the skin color of chicken shanks and an application thereof. Background technique [0002] Chicken is a kind of poultry, which is the main edible poultry in our country. Consumers largely determine how chickens are bred and sold. For example, in southern China, whole chickens are mainly sold, while in northern China, they are mainly sold in splits. People in different regions have different preferences for broiler skin color, but the golden color of broiler chicken is considered to be closely related to the quality of meat. Consumers are more inclined to buy broiler chickens with good appearance (yellow skin) and consistent appearance characteristics. Due to several outbreaks of bird flu, the sales of fresh chickens have been restricted, and their sales have gradually declined, while the market for chilled chickens has gradually expanded,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 罗文吴静文张细权聂庆华
Owner SOUTH CHINA AGRI UNIV
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