MTC dye-based visual detection method of blood unbound transferrin and application thereof

Abstract

The invention discloses an MTC dye-based method for detecting blood unbound transferrin, and belongs to the technical field of clinical detection methods. An MTC dye is mixed with blood to be detected; when unbound ferritin in the blood is increased, the MTC aggregation form can be induced to be converted into J-aggregate; therefore, the color of the to-be-detected blood becomes blue; there is a maximum absorbance peak value at 660 nm, change of the concentration of the unbound ferritin in the blood is judged according to change of the color of the blood to be detected, and the concentration of the unbound ferritin in the blood is calculated according to the value of the absorbance of the blood to be detected, so that detection of the unbound ferritin in the blood is realized. According tothe detection method, only one MTC detection substance needs to be used, the steps are simple and convenient, interference factors are few, and therefore a detection result is stable. Meanwhile, according to the detection method disclosed by the invention, visual detection of the blood unbound ferritin based on the MTC dye is realized along with the visible color change.

Description

technical field [0001] The invention belongs to the technical field of clinical detection methods, and relates to a visual detection method and application of MTC dye-based blood unbound transferrin. Background technique [0002] 3,3'-dithiopropyl-4,5,4',5'-dibenzo-9-methylthiocarbocyanine triethylammonium salt (MTC) is a large class of functional fluorescent It is very sensitive to environmental changes, has various aggregation states, and different aggregates can be visualized due to large differences in optical properties. It can self-assemble with biological macromolecules as templates and the process is regulated by the structure of biological macromolecules. Therefore, in solution And in some matrices, they can self-assemble into nanoscale supramolecular aggregates. By utilizing the interconversion between MTC aggregates to reflect certain structural properties of biomacromolecules or as a probe for specific recognition of biomolecules. Therefore, the structural stat...

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Problems solved by technology

[0006] In order to overcome the above-mentioned shortcoming of the prior art, the object of the present invention is to provide a kind of blood unconjugated transferrin ferritin detection method, detection kit and probe based on MTC dye, to solve the complex operation and interference factors of the existing detection method More problems that lead to unstable test results

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