Peanut protein characteristic peptide and its application

A peanut protein and characteristic peptide technology, which is applied in the direction of peptide source, plant peptide, fat production, etc., can solve the problems of reduced degree of denaturation, low residual oil rate of peanut kernel residue, failure to achieve quality control standards, etc., and achieve the goal of eliminating interfering ions Effects of impact, high resolution, increased reliability and accuracy

Active Publication Date: 2021-03-26
青岛吴昊植物油有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The most important thing is that the residual oil rate in the by-product peanut kernel residue is low, and the protein nitrogen solubility index is 73.58%, which is much higher than the level of 11.6% in ordinary pressing, and the degree of denaturation is significantly reduced. Peanut protein powder and peanut protein can be prepared through reprocessing Milk, peanut butter and other products, but the existing technology lacks corresponding means to detect the allergens, active ingredients of peanut protein, etc., and cannot achieve quality control standards that can be indexed
Moreover, in the prior art, there is also a lack of effective detection means for the content of effective peanut ingredients in products such as peanut protein powder, peanut protein milk, and peanut butter.

Method used

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  • Peanut protein characteristic peptide and its application
  • Peanut protein characteristic peptide and its application
  • Peanut protein characteristic peptide and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 The process of extracting peanut oil with fried embryos and the preparation of corresponding by-products

[0026] A kind of production technology of peanut oil by-product of fried embryo water extraction, it is characterized in that, the production technology of described fried embryo water extraction peanut oil comprises the following steps:

[0027] (1) Select the raw material, remove the peanut red coat, and crush the peanut petals to 3-6 petals;

[0028] (2) Stir-fry the peanut petals at 220-230°C for 20-30 minutes;

[0029] (3) Adjust the temperature and moisture of the stir-fried peanut petals to soften the peanut petals. The softened peanut petals have a moisture content of 7-8% and a temperature of 70-85°C;

[0030] (4) Send the softened peanut petals into an oil press for pressing to obtain virgin peanut oil and peanut residues. The temperature for pressing is 70-85°C, and the treatment is carried out at 5-10Mpa for 40-60min, and then at 110- Treat ...

Embodiment 2

[0034] The establishment of embodiment 2 detection system

[0035] The instrument equipment, chemical reagent that the present invention adopts, specifically as follows:

[0036] Ultra-high performance liquid chromatography tandem time-of-flight mass spectrometry (UPLC-Q-TOF, ThermoFisher, USA); 5427R desktop high-speed refrigerated centrifuge: Eppendorf, Germany; high-temperature drying oven: Shanghai Jinghong Experimental Equipment Co., Ltd.; CF15RXII centrifuge: Hitachi, Japan Company; Y905 pulping machine: Joyoung Co., Ltd.; XS205 balance: Mettler-Toledo, Switzerland; 10ku ultrafiltration centrifuge tube: Sartorius, Germany.

[0037] Acetonitrile: chromatographically pure, Fisher Company of the United States; formic acid: chromatographically pure, Tedia Company of the United States; dithiothreitol (DTT) (biochemical grade), sequencing grade trypsin: Promega Company of the United States; iodoacetamide (IAA): biochemical grade , Sigma Corporation of the United States; prote...

Embodiment 3

[0057] The detection of embodiment 3 sample

[0058] Select the by-products of fried embryo water extraction peanuts and other samples in Example 1 (including dried peanuts (Luhua No. 28, Shanyou No. 523, Yueyou No. 13, Yuanza No. 9102), a certain brand of peanut candy, on the package Two parts of biscuits without peanuts as raw materials, peanut milk of a certain brand, peanut meal obtained by ordinary high-temperature pressing, and commercial peanut meal of a certain brand) were used as research objects to determine the peanut protein content in the samples

[0059] (1) Take 50 μL of the characteristic peptide solution (10 μg / mL), add 15 μL of isotopic formaldehyde solution (4%) and 15 μL of sodium cyanoborohydride solution (0.6mol / L), vortex for 5-30 seconds, mix thoroughly and then room temperature After standing still, 1 ml of ammonia water (1%) and 50 μL of formic acid were added to terminate the reaction, and left at room temperature for 1 hour to obtain an internal sta...

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Abstract

The invention belongs to the field of biological detection of protein markers, and in particular relates to a peanut protein characteristic peptide and its application. The amino acid sequence of the characteristic peptide is ENEGGNIFSG. The present invention screens out characteristic peptides by means of ultra-high performance liquid chromatography tandem time-of-flight mass spectrometry, designs and synthesizes corresponding internal standards, and uses high performance liquid chromatography coupled with mass spectrometry to calculate and obtain the peanut allergen protein in peanut processing by-products The content of peanut protein can be further obtained, and the method has good linearity, sensitivity, recovery and precision.

Description

technical field [0001] The present invention relates to a characteristic peptide of peanut protein and its application, in particular to a characteristic peptide for extracting by-products of peanut oil, in particular to a characteristic peptide derived from peanut Arah3 / 4 protein, and its application in peanut production and processing . Background technique [0002] Peanut is an important oil crop and economic crop in my country. Its oil content is between 46% and 62%, most of which are unsaturated fatty acids. Peanut oil has a delicate smell, pure taste and rich nutrition. It is currently the main oil resource in our country. At present, the extraction methods of peanut oil in my country include pressing method, solvent leaching method, aqueous method, and aqueous enzymatic method. Except that the aqueous enzymatic method has relatively small defects, although the other three methods have their own advantages, their defects are also obvious. In the oil production proces...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): C07K14/415G01N30/02G01N30/06G01N30/72G01N30/86C11B1/04C11B1/06C11B3/00
CPCC07K14/415C11B1/04C11B1/06C11B3/00G01N30/02G01N30/06G01N30/72G01N30/8675G01N2030/045
Inventor吴海鹏
Owner青岛吴昊植物油有限公司