55 results about "Peanut allergy" patented technology

An allergen-free transgenic peanut seed is produced by recombinant methods. Peanut plants are transformed with multiple copies of each of the allergen genes, or fragments thereof, to suppress gene expression and allergen protein production. Alternatively, peanut plants are transformed with peanut allergen antisense genes introduced into the peanut genome as antisense fragments, sense fragments, or combinations of both antisense and sense fragments. Peanut transgenes are under the control of the 35S promoter, or the promoter of the Ara h2 gene to produce antisense RNAs, sense RNAs, and double-stranded RNAs for suppressing allergen protein production in peanut plants. A full length genomic clone for allergen Ara h2 is isolated and sequenced. The ORF is 622 nucleotides long. The predicted encoded protein is 207 amino acids long and includes a putative transit peptide of 21 residues. One polyadenilation signal is identified at position 951. Six additional stop codons are observed. A promoter region was revealed containing a putative TATA box located at position −72. Homologous regions were identified between Ara h2, h6, and h7, and between Ara h3 and h4, and between Ara h1P41B and Ara h1P17. The homologous regions will be used for the screening of peanut genomic library to isolate all peanut allergen genes and for down-regulation and silencing of multiple peanut allergen genes.

The invention relates to the field of biotechnology, in particular to a promoter Arah1-P of a peanut allergen gene Arah1 and its cloning and application. The nucleotide sequence of the promoter Arah1-P of the above-mentioned peanut allergen gene Arah1 is shown in SEQ IN NO: 1. The promoter Arah1-P of the peanut allergen gene Arah1 can drive the specific expression of foreign genes in plant seeds. The plant expression vector of the promoter Arah1-P of the above-mentioned peanut allergen gene Arah1 is pBI121-Arah1-P. The promoter Arah1-P of the peanut allergen gene Arah1 of the present invention has important application value for improving the quality of peanut seeds and researching peanut seeds as "bioreactor".

The invention belongs to the field of biological detection of protein markers, and in particular relates to a peanut protein characteristic peptide and its application. The amino acid sequence of the characteristic peptide is ENEGGNIFSG. The present invention screens out characteristic peptides by means of ultra-high performance liquid chromatography tandem time-of-flight mass spectrometry, designs and synthesizes corresponding internal standards, and uses high performance liquid chromatography coupled with mass spectrometry to calculate and obtain the peanut allergen protein in peanut processing by-products The content of peanut protein can be further obtained, and the method has good linearity, sensitivity, recovery and precision.

The present invention relates to a poxvirus vector comprising a nucleic acid sequence encoding a fusion protein comprising: (i) a peanut allergen selected from list consisting of at least two peanut allergens from ara h 1, ara h 2, ara h 3, ara h 4, ara h 5, ara h 6. ara h 7, ara h 8, ara h 9, ara h I O and ara h I I or a derivative or part thereof having at least 70% sequence identity thereto, and (ii) a profeasome degradation tag to enhance intracellular degradation of the fusion protein. Methods of desensitizing or inducing tolerance to a peanut allergen and / or suppressing an allergic response to a peanut allergen are also disclosed.