Recombinant peanut allergen and mutant and preparation method and application of recombinant peanut allergen and mutant

A technology of peanut allergens and mutants, applied in the field of genetic engineering, can solve the problems of large manpower and financial resources consumption, wide application limitations, complex extract components, etc., and achieve the effect of reducing the binding ability
CN102584966AInactive Publication Date: 2012-07-18THE SECOND AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
THE SECOND AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV
Publication Date
2012-07-18
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a recombinant peanut allergen and a mutant and a preparation method and application of the recombinant peanut allergen and the mutant. The recombinant peanut allergen is a non-natural mutant derived from a natural allergen, and the amino acid sequence of the recombinant peanut allergen is shown as SEQ ID NO: 1. A coding gene of the recombinant peanut allergen is artificially synthesized after codon optimization by using a gene engineering technology; and the epitope of the coding gene is analyzed by adopting biological informatics software, and directional displacement is performed on one or more sites with high antigenicity by a site-directed mutagenesis method to reduce the allergenicity of the allergen, and thus the recombinant peanut allergen mutant with low allergenicity is constructed. The recombinant peanut allergen with high purity and a mutant protein of the allergen are obtained by protein expression under an artificial controlled condition. Compared with a natural peanut allergen, the invention has the advantages that the bonding capacity of the recombinant protein mutant bonded with specific immunoglobulin E (IgE) is obviously reduced, and the recombinant protein mutant can be safely applied to diagnosis and treatment of allergic diseases and even judgment of other protein allergenicity.
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Description

technical field

[0001] The invention relates to the field of genetic engineering, in particular to a recombinant peanut allergen and mutant, a preparation method and application thereof. Background technique

[0002] Peanuts belong to leguminous plants with rich nutritional value and relatively balanced amino acid content. It is reported that peanuts contain 45-53% oil and 24-29% protein. In recent years, allergic diseases have received attention from all walks of life, and peanut is one of the eight major allergic foods announced in the report of the Food and Agriculture Organization of the United Nations (FAO), and it is also a very important food allergen. 10%-47% of the total number of allergic cases, and the clinical symptoms of peanut allergy are severe, even eating a small amount of peanut allergen food can trigger a strong allergic reaction, which has attracted much attention in the fields of public health and food safety. On the other hand, due to the prevalence an...

Claims

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