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A promoter arah1-p of peanut allergen gene arah1 and its cloning and application

A peanut allergen and promoter technology, applied in application, genetic engineering, plant genetic improvement and other directions, can solve the problems of lack of peanut promoter resources and unidentified promoter functions.

Active Publication Date: 2021-12-07
SHANDONG PEANUT RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, currently available peanut promoter resources are relatively scarce, and most promoter functions have not been identified

Method used

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  • A promoter arah1-p of peanut allergen gene arah1 and its cloning and application
  • A promoter arah1-p of peanut allergen gene arah1 and its cloning and application
  • A promoter arah1-p of peanut allergen gene arah1 and its cloning and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] This embodiment specifically includes the following test process:

[0016] 1.1 Test material

[0017] The plant materials were peanut variety "Huayu 9303" and Arabidopsis thaliana (ecotype Co10). Escherichia coli DH5α competence, DNA molecular weight markers, PCR mix, etc. were purchased from Beijing Quanshijin Biological Co., Ltd.; high-fidelity enzyme Phusion was purchased from NewEngland Biolabs; X-Gluc was purchased from Beijing Suolaibao Technology Co., Ltd.; restriction endonuclease Enzymes BamHI and HindIII were purchased from Fermentas; T4 DNA ligase was purchased from Bao Biological Engineering (Dalian) Co., Ltd.; plasmid mini-extraction kit and gel recovery kit were purchased from Tiangen Biochemical Technology (Beijing) Co., Ltd.; Agrobacterium strain GV3101 And the overexpression vector pBI121 is preserved in our laboratory.

[0018] 1.2 Arah1 gene endogenous expression analysis

[0019] Gene-specific primers QF and QR (as shown in Table 1) were designed ...

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Abstract

The invention relates to the field of biotechnology, in particular to a promoter Arah1-P of a peanut allergen gene Arah1 and its cloning and application. The nucleotide sequence of the promoter Arah1-P of the above-mentioned peanut allergen gene Arah1 is shown in SEQ IN NO: 1. The promoter Arah1-P of the peanut allergen gene Arah1 can drive the specific expression of foreign genes in plant seeds. The plant expression vector of the promoter Arah1-P of the above-mentioned peanut allergen gene Arah1 is pBI121-Arah1-P. The promoter Arah1-P of the peanut allergen gene Arah1 of the present invention has important application value for improving the quality of peanut seeds and researching peanut seeds as "bioreactor".

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a promoter Arah1-P of peanut allergen gene Arah1 and its cloning and application. Background technique [0002] Peanut is an important oil crop in the world. Its seeds are rich in fat and protein, and it is an ideal "bioreactor". Promoters can drive the expression of foreign genes in host plants, and are important molecular tools for peanut genetic improvement. Since the acquisition of genetically modified peanuts in 1993, there have been reports of genetically modified peanuts aimed at stress resistance and quality improvement. However, currently available peanut promoter resources are relatively scarce, and the functions of most promoters have not been identified. [0003] Peanut is one of the eight major allergic foods in the world. At present, 17 kinds of peanut allergen proteins have been found. Among them, the peanut allergen gene Arah1 has the highest protein content and is...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113C12N15/66C12N15/84A01H5/10A01H6/20A01H6/54
CPCC07K14/415C12N9/2402C12N15/66C12N15/8234C12Y302/01031
Inventor 苑翠玲孙全喜单世华闫彩霞赵小波王娟李春娟张浩
Owner SHANDONG PEANUT RES INST
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