Traditional Chinese medicine extract composition for treating breast cancer, preparation method and application of traditional Chinese medicine extract composition in preparation of drugs

A technology of extracts and compositions, which is applied in the field of composition preparation, can solve problems that have not yet appeared in research, and achieve the effects of reducing clinical doses, reducing toxic and side effects, and optimizing the ratio of extracts

Inactive Publication Date: 2021-03-23
杭州微扁担智能科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Research on the composition of Coix seed and Poria cocos extracts has not yet appeared

Method used

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  • Traditional Chinese medicine extract composition for treating breast cancer, preparation method and application of traditional Chinese medicine extract composition in preparation of drugs
  • Traditional Chinese medicine extract composition for treating breast cancer, preparation method and application of traditional Chinese medicine extract composition in preparation of drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The preparation of embodiment 1 Chinese medicine extract

[0021] 1. Drug raw materials

[0022] Coix seed, Poria cocos.

[0023] 2. Extract preparation method

[0024] Coix seed extract: Coix seed is crushed into 12-mesh coarse powder, soaked for 1 hour, extracted with water for 3 times at 80-90°C, 1 hour each time, 10 times the water, and the stirring speed is 20 rpm during the extraction process. Combine the extracts, filter, centrifuge at 3000r / min for 10 minutes, take the supernatant, concentrate to contain 1g crude drug per ml, and set aside;

[0025] Poria cocos extract: Poria cocos medicinal material is crushed into 12-mesh coarse powder, soaked for 1 hour, extracted with water for 3 times at 80-90°C, 1 hour each time, 10 times of water, stirring speed 20rpm during the extraction process. Combine the extracts, filter, centrifuge at 3000r / min for 10 minutes, take the supernatant, concentrate to contain 1g crude drug per ml, and set aside.

Embodiment 2

[0026] Example 2 The synergistic effect of the combination of extracts on inhibiting breast cancer cells

[0027] 1. Experimental materials

[0028] (1) Drugs: coix seed extract, Poria cocos extract.

[0029] (2) Breast cancer cells: human breast cancer cells mcf-7 cells.

[0030] (3) Commercially available MTT kits.

[0031] 2. Experimental grouping

[0032] (1) Concentration gradient of coix seed extract.

[0033] (2) Poria cocos extract concentration gradient.

[0034] (3) Coix seed extract and Poria cocos extract are combined in equal proportions.

[0035] 3. MTT method to detect the sensitivity of tumor cells to different traditional Chinese medicine extracts

[0036] (1) Human breast cancer cell mcf-7 cells were inoculated into 96-well plates at the number of 5000-9000 cells per well. After the cells adhered to the wall, different crude drug concentrations of Coix seed extract, Poria combined PBS solution. After culturing for 24 hours, add 10 μl of MTT with a con...

Embodiment 3

[0038] Example 3 Effects of Different Combination Ratio of Chinese Medicine Extracts on Tumor Cell Inhibition

[0039] 1. Experimental materials

[0040] (1) Drugs: coix seed extract, Poria cocos extract.

[0041] (2) Breast cancer cells: human breast cancer cells mcf-7 cells.

[0042] (3) Commercially available MTT kits.

[0043] 2. Experimental grouping

[0044] Coix seed extract and Poria cocos extract are combined in different proportions.

[0045] 3. MTT method to detect the sensitivity of tumor cells to different traditional Chinese medicine extracts

[0046] (1) Human breast cancer cell mcf-7 cells were inoculated into 96-well plates at the number of 5000-9000 cells per well. After the cells adhered to the wall, different crude drug concentrations of Coix seed extract and Poria cocos extract were added respectively. PBS solution. After culturing for 24 hours, add 10 μl of MTT with a concentration of 5 mg / ml to each well for another 4 hours, then discard the cultur...

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Abstract

The invention relates to a traditional Chinese medicine extract composition for treating a breast cancer, a preparation method and application of the traditional Chinese medicine extract composition in preparation of drugs. Raw pharmaceutical material extracts serve as various ingredients, wherein a weight ratio of a coix seed extract to a poria cocos extract is (1 to 15): (1 to 15). The coix seedextract is an aqueous extract; and the poria cocos extract is an aqueous extract. Distinguished from the traditional viewpoint that traditional Chinese medicine compounded co-decoction is superior toseparate extracting of all medicinal materials, the invention proposes an optimal combination method for all medicinal material extracts, accurate control on the composition is achieved, pharmacological effects are improved remarkably, and the method has a positive guiding action on treatment methods for the breast cancer by the traditional Chinese medicine extract composition.

Description

technical field [0001] The invention relates to a composition of traditional Chinese medicine extracts for treating breast cancer, a preparation method of the composition for treating breast cancer, and an application of the composition for treating breast cancer in preparing medicines for treating breast cancer. Background technique [0002] Breast cancer is one of the most common malignant tumors in women. According to statistics, the incidence rate accounts for 7-10% of all kinds of malignant tumors in the whole body. It has become the number one cancer killer threatening women's health. The cause of breast cancer has not been fully clarified However, reproductive status, oral contraceptives, genetic factors, ionizing radiation, unhealthy eating habits and other factors have been confirmed to be related to breast cancer. Although the mammary gland is not an important organ to maintain human life activities, breast cancer in situ is not fatal; but because breast cancer cel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8994A61P35/00
CPCA61K36/076A61K36/8994A61K2236/331A61K2236/39A61P35/00A61K2300/00
Inventor 李斌
Owner 杭州微扁担智能科技有限公司
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