LC-MS/MS method for quantitatively analyzing plasma concentration of antituberculous drug

Abstract

The invention discloses an LC-MS / MS method for quantitatively analyzing the plasma concentration of an antituberculous drug. The method can simultaneously detect isoniazide, rifampicin, pyrazinamide and ethambutol through S1 standard working solution preparation, S2 sample preparation, S3 sample treatment, S4 sample detection and S5 standard curve making and quantitative analysis, the sample pretreatment process is simple and is easy to operate, and all the reagents are conventional reagents, so that the cost is lower. According to the method, a reversed-phase C18 column of 150 mm*2.1 mm is adopted, the flow rate is controlled to be 0.3 mL / min, and the column temperature is 30 DEG C; the problem that the polarity difference of isoniazide, rifampicin, pyrazinamide and ethambutol is large can be well solved, the separation effect is better, and the cost is lower. The method has the advantages of higher sensitivity, accuracy and precision, wide detection linear range and short detection time, and can be used as a reliable detection method suitable for popularization and for monitoring the treatment concentration of clinical first-line anti-tuberculosis drugs.

Description

technical field [0001] The invention belongs to the technical field of drug analysis, in particular to an LC-MS / MS method for quantitatively analyzing plasma concentrations of anti-tuberculosis drugs. Background technique [0002] Tuberculosis is an infectious disease caused by patients infected with Mycobacterium tuberculosis. The current clinical treatment plan is the long-term combined application of various anti-tuberculosis drugs, among which rifampicin (RFP), isoniazid (Isoniazid, INH) , Pyrazinamide (PZA) and ethambutol (Ethambutol, EMB) belong to the first-line anti-TB drugs. Because tuberculosis patients are usually combined with other diseases and use multiple drugs at the same time, coupled with individual differences in patients, the concentration of anti-tuberculosis drugs in the human body varies greatly. If the drug concentration in the body is too low, the therapeutic effect cannot be achieved; if the drug concentration in the body is too high, it will cause...

AI Technical Summary

Problems solved by technology

[0003] At present, there are mainly liquid chromatography, liquid phase-tandem mass spectrometry (LC-MS / MS) and ultra-high performance liquid phase-tandem mass spectrometry (UPLC-MS / MS) methods for detecting the plasma concentration of anti-tuberculosis drugs at home and abroad. In some methods, only one anti-tuberculosis drug is detected, the detection time is long, and the detection component is single, which is not suitable for combined drug use

Some methods are to detect multiple anti-tuberculosis drugs at the same time, and there are even patent reports to detect 11 kinds of anti-tuberculosis drugs at the same time, but most of the methods for simultaneous detection of these drugs use UPLC-MS / MS method, which requires the use of UPLC liquid chromatography and UPLC column, the detection cost is relatively high, and it is not suitable for promotion

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